Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA3 CRISPR/Cas9 KO Plasmid (h): sc-404634
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxA3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA3 Antibody (F-7): sc-374237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA3 CRISPR/Cas9 KO Plasmid (h) | sc-404634 | 20 µg | $397.00 |
Overview
HOXA3 encodes the homeobox transcription factor HoxA3, a sequence-specific DNA-binding regulator that patterns anterior–posterior identity during embryogenesis and coordinates programs governing morphogenesis, epithelial differentiation, and tissue remodeling. HoxA3 integrates with HOX cluster regulatory networks and intersects with developmental signaling pathways such as WNT, BMP/TGF-β, and retinoic acid to shape cell fate decisions and spatial gene expression. In adult contexts, altered HOXA3 expression or epigenetic dysregulation has been linked to aberrant differentiation states and transcriptional rewiring observed in cancer and other disorders involving developmental gene reactivation. As a nuclear regulator, HoxA3 is frequently studied in gene regulatory network mapping, lineage specification models, and chromatin-mediated control of transcription.
HoxA3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXA3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXA3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXA3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxA3 protein expression.
This CRISPR knockout system enables efficient generation of HOXA3-deficient cell models for investigation of HoxA3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXA3 exon(s) critical for HoxA3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXA3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxA3 CRISPR/Cas9 KO Plasmid (h) and HoxA3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXA3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxA3 HDR Plasmid (h) and HoxA3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXA3 homology arms to support homology-directed repair at defined HOXA3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.