Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA10 CRISPR/Cas9 KO Plasmid (m): sc-420900
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxA10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA10 Antibody (E-11): sc-271428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA10 CRISPR/Cas9 KO Plasmid (m) | sc-420900 | 20 µg | $397.00 |
Overview
Hoxa10 encodes the homeobox transcription factor HoxA10, a DNA-binding regulator that patterns the embryonic anterior–posterior axis and specifies regional identity in the developing urogenital and reproductive tracts. In mouse, HoxA10 contributes to developmental gene regulatory networks by coordinating transcriptional programs that control cell fate determination, differentiation, and morphogenesis, with context-dependent effects on proliferation and tissue remodeling. Its activity interfaces with broader HOX/TALE cofactors and chromatin regulation to establish segment-specific transcriptional states. Dysregulated HOXA10 expression has been associated with altered hematopoietic differentiation and leukemogenic transcriptional programs, supporting its use as a mechanistic node in models of developmental disorders and cancer-relevant lineage specification.
HoxA10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hoxa10 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hoxa10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hoxa10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxA10 protein expression.
This CRISPR knockout system enables efficient generation of Hoxa10-deficient cell models for investigation of HoxA10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hoxa10 exon(s) critical for HoxA10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hoxa10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxA10 CRISPR/Cas9 KO Plasmid (m) and HoxA10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hoxa10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxA10 HDR Plasmid (m) and HoxA10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hoxa10 homology arms to support homology-directed repair at defined Hoxa10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.