Hox11 CRISPR/Cas9 KO Plasmid (m): sc-423423
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hox11 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hox11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hox11 Antibody (1D7): sc-12760
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hox11 CRISPR/Cas9 KO Plasmid (m) | sc-423423 | 20 µg | $397.00 |
Overview
Tlx1 (Hox11) is a TALE-class homeobox transcription factor that regulates embryonic patterning and organogenesis, with prominent roles in splenic development and hematopoietic lineage specification in mouse. By binding sequence-specific regulatory elements and partnering with cofactors such as PBX/MEIS proteins, Hox11 modulates transcriptional programs controlling progenitor proliferation, differentiation, and tissue compartmentalization. Dysregulated TLX1/Hox11 activity is associated with aberrant developmental programs and is implicated in oncogenic transcriptional networks, particularly in T-lineage leukemogenesis models. In experimental systems, Tlx1 provides a tractable entry point for dissecting transcriptional control of developmental gene networks and their rewiring in transformation.
Hox11 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tlx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tlx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tlx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hox11 protein expression.
This CRISPR knockout system enables efficient generation of Tlx1-deficient cell models for investigation of Hox11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tlx1 exon(s) critical for Hox11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tlx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hox11 CRISPR/Cas9 KO Plasmid (m) and Hox11 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tlx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hox11 HDR Plasmid (m) and Hox11 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tlx1 homology arms to support homology-directed repair at defined Tlx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.