Homer Antibody (D-3): sc-17842
- Homer Antibody (D-3) is a mouse monoclonal IgG1 κ Homer antibody, cited in 24 publications, provided at 200 µg/ml
- raised against amino acids 1-354 of Homer of human origin; requires amino acids 13-181 for recognition
- Homer Antibody (D-3) is recommended for detection of Homer of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Homer Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Homer Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Homer Antibody (D-3) (see ordering information below).
Homer Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that specifically recognizes Homer protein of mouse, rat, and human origin in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Homer proteins, also known as Vesl proteins (VASP/Ena-related gene upregulated during seizure), are a family of scaffold proteins that play a crucial role in synaptic signaling by binding to group 1 metabotropic glutamate receptors (mGluRs), which are involved in triggering intracellular calcium release. The interaction between Homer proteins and mGluRs is essential for synaptic plasticity and neurotransmission, affecting learning and memory processes. Homer-1a, an immediate early gene product induced by neuronal activity, differs from other constitutively expressed Homer proteins (such as Homer-1b, -1c, -2, and -3) by lacking a coiled-coil domain, preventing multimerization and modulating synaptic signaling pathways. By linking mGluRs to intracellular calcium channels like the inositol triphosphate receptors (IP3R), Homer proteins facilitate the regulation of intracellular calcium dynamics, which is vital for numerous neuronal functions. Understanding the function and regulation of Homer proteins is important for research into neurological disorders and synaptic dysfunctions. Homer (D-3) monoclonal antibody is available in both unconjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, making it versatile for different experimental needs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Homer Antibody (D-3) References:
- Mouse brain and muscle tissues constitutively express high levels of Homer proteins. | Soloviev, MM., et al. 2000. Eur J Biochem. 267: 634-9. PMID: 10651798
- Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing. | Soloviev, MM., et al. 2000. J Mol Biol. 295: 1185-200. PMID: 10653696
- Homer-3 regulates activation of serum response element in T cells via its EVH1 domain. | Ishiguro, K. and Xavier, R. 2004. Blood. 103: 2248-56. PMID: 14645007
- Homer: a protein that selectively binds metabotropic glutamate receptors. | Brakeman, PR., et al. 1997. Nature. 386: 284-8. PMID: 9069287
- vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis. | Kato, A., et al. 1997. FEBS Lett. 412: 183-9. PMID: 9257717
- Novel members of the Vesl/Homer family of PDZ proteins that bind metabotropic glutamate receptors. | Kato, A., et al. 1998. J Biol Chem. 273: 23969-75. PMID: 9727012
- Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins. | Xiao, B., et al. 1998. Neuron. 21: 707-16. PMID: 9808458
- Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors. | Tu, JC., et al. 1998. Neuron. 21: 717-26. PMID: 9808459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Homer Antibody (D-3) | sc-17842 | 200 µg/ml | $316.00 | |||
Homer Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-528333 | 200 µg Ab; 10 µg BP | $354.00 | |||
Homer Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-520681 | 200 µg Ab, 40 µg BP | $354.00 | |||
Homer Antibody (D-3): m-IgG1 BP-HRP Bundle | sc-542871 | 200 µg Ab; 20 µg BP | $354.00 | |||
Homer Antibody (D-3) AC | sc-17842 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Homer Antibody (D-3) HRP | sc-17842 HRP | 200 µg/ml | $316.00 | |||
Homer Antibody (D-3) FITC | sc-17842 FITC | 200 µg/ml | $330.00 | |||
Homer Antibody (D-3) PE | sc-17842 PE | 200 µg/ml | $343.00 | |||
Homer Antibody (D-3) Alexa Fluor® 488 | sc-17842 AF488 | 200 µg/ml | $357.00 | |||
Homer Antibody (D-3) Alexa Fluor® 546 | sc-17842 AF546 | 200 µg/ml | $357.00 | |||
Homer Antibody (D-3) Alexa Fluor® 594 | sc-17842 AF594 | 200 µg/ml | $357.00 | |||
Homer Antibody (D-3) Alexa Fluor® 647 | sc-17842 AF647 | 200 µg/ml | $357.00 | |||
Homer Antibody (D-3) Alexa Fluor® 680 | sc-17842 AF680 | 200 µg/ml | $357.00 | |||
Homer Antibody (D-3) Alexa Fluor® 790 | sc-17842 AF790 | 200 µg/ml | $357.00 |