Homer-3 Antibody (E-2): sc-376154

Homer-3 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Homer-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Homer-3 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Homer-3 protein plays a crucial role in synaptic signaling by binding to group 1 metabotropic glutamate receptors (mGluRs), which are essential for mediating intracellular calcium release and synaptic plasticity. This function is particularly important in learning and memory, as mGluRs are involved in long-term potentiation (LTP), a process that strengthens synapses based on recent patterns of activity. Unlike Homer-1a, which is transiently expressed and inhibits multimerization of other Homer proteins, Homer-3 is constitutively expressed and contains a coiled-coil domain that facilitates self-multimerization, thereby enhancing binding capacity to link mGluRs with inositol triphosphate receptors (IP3R). This interaction is vital for regulating calcium signaling pathways, making Homer-3 a significant player in neuronal communication and plasticity.

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Homer-3 Antibody (E-2) References:

1. Mouse brain and muscle tissues constitutively express high levels of Homer proteins.  |  Soloviev, MM., et al. 2000. Eur J Biochem. 267: 634-9. PMID: 10651798
2. Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing.  |  Soloviev, MM., et al. 2000. J Mol Biol. 295: 1185-200. PMID: 10653696
3. Homer-3 regulates activation of serum response element in T cells via its EVH1 domain.  |  Ishiguro, K. and Xavier, R. 2004. Blood. 103: 2248-56. PMID: 14645007
4. A screen for proteins that interact with PAX6: C-terminal mutations disrupt interaction with HOMER3, DNCL1 and TRIM11.  |  Cooper, ST. and Hanson, IM. 2005. BMC Genet. 6: 43. PMID: 16098226
5. Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission.  |  Rezvani, K., et al. 2012. J Neurochem. 122: 24-37. PMID: 22486777
6. Patient with homer-3 antibodies and cerebellitis.  |  Höftberger, R., et al. 2013. JAMA Neurol. 70: 506-9. PMID: 23400636
7. 'Medusa-head ataxia': the expanding spectrum of Purkinje cell antibodies in autoimmune cerebellar ataxia. Part 1: Anti-mGluR1, anti-Homer-3, anti-Sj/ITPR1 and anti-CARP VIII.  |  Jarius, S. and Wildemann, B. 2015. J Neuroinflammation. 12: 166. PMID: 26377085
8. Homer: a protein that selectively binds metabotropic glutamate receptors.  |  Brakeman, PR., et al. 1997. Nature. 386: 284-8. PMID: 9069287
9. vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis.  |  Kato, A., et al. 1997. FEBS Lett. 412: 183-9. PMID: 9257717
10. Novel members of the Vesl/Homer family of PDZ proteins that bind metabotropic glutamate receptors.  |  Kato, A., et al. 1998. J Biol Chem. 273: 23969-75. PMID: 9727012
11. Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins.  |  Xiao, B., et al. 1998. Neuron. 21: 707-16. PMID: 9808458
12. Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors.  |  Tu, JC., et al. 1998. Neuron. 21: 717-26. PMID: 9808459

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Homer-3 Antibody (E-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Homer-3 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Homer-3 Antibody (E-2) Specifications

Alternate Product Name: HOMER3

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 228-361

Epitope Target: Homer-3

Epitope Species: human

Product Reactivity/Detection: Homer-3a and Homer-3b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Homer-3 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Homer antibody is Homer Antibody (D-3): sc-17842.