Hog1 Antibody (D-3): sc-165978

Hog1 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hog1 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hog1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hog1 plays a crucial role in the high-osmolarity glycerol (HOG) signaling pathway, which is essential for yeast cells to adapt to osmotic stress. Located primarily in the cytoplasm, anti-Hog1 antibody (D-3) can detect Hog1′s translocation to the nucleus upon activation, where Hog1 regulates the expression of genes involved in osmotic stress response. This translocation enables Hog1 to modulate the transcription of osmo-regulatory proteins, thereby helping cells maintain homeostasis under varying environmental conditions. Hog1′s interactions with various transcription factors and other signaling molecules underscore its importance in cellular adaptation and survival, making anti-Hog1 antibody (D-3) an invaluable tool for researchers studying stress response mechanisms in yeast.

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Hog1 Antibody (D-3) References:

1. PBS2, a yeast gene encoding a putative protein kinase, interacts with the RAS2 pathway and affects osmotic sensitivity of Saccharomyces cerevisiae.  |  Boguslawski, G. 1992. J Gen Microbiol. 138: 2425-32. PMID: 1479360
2. Hog1 bypasses stress-mediated down-regulation of transcription by RNA polymerase II redistribution and chromatin remodeling.  |  Nadal-Ribelles, M., et al. 2012. Genome Biol. 13: R106. PMID: 23158682
3. The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress.  |  Regot, S., et al. 2013. J Biol Chem. 288: 17384-98. PMID: 23645671
4. Thiol-based direct threat sensing by the stress-activated protein kinase Hog1.  |  Guerra-Moreno, A., et al. 2019. Sci Signal. 12: PMID: 31772124
5. The coordinate actions of calcineurin and Hog1 mediate the stress response through multiple nodes of the cell cycle network.  |  Leech, CM., et al. 2020. PLoS Genet. 16: e1008600. PMID: 32343701
6. Crosstalk between Saccharomycescerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation.  |  Laz, EV., et al. 2020. Fungal Biol. 124: 361-367. PMID: 32389298
7. Hog1-induced transcription of RTC3 and HSP12 is robust and occurs in cells lacking Msn2, Msn4, Hot1 and Sko1.  |  Bai, C., et al. 2020. PLoS One. 15: e0237540. PMID: 32804965
8. The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene.  |  Schüller, C., et al. 1994. EMBO J. 13: 4382-9. PMID: 7523111
9. Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor.  |  Maeda, T., et al. 1995. Science. 269: 554-8. PMID: 7624781
10. An osmosensing signal transduction pathway in yeast.  |  Brewster, JL., et al. 1993. Science. 259: 1760-3. PMID: 7681220
11. MAP kinase pathways in yeast: for mating and more.  |  Herskowitz, I. 1995. Cell. 80: 187-97. PMID: 7834739
12. Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 'two-component' osmosensor.  |  Posas, F., et al. 1996. Cell. 86: 865-75. PMID: 8808622

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hog1 Antibody (D-3) has 21 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hog1 Antibody (D-3) Specifications

Alternate Product Name: Hog1

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 291-408

Epitope Target: Hog1

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Hog1

Species Reactivity/Detection: y

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for Hog1 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin