Hog1 Antibody (A-8): sc-165977

Hog1 Antibody (A-8) is a mouse monoclonal IgG2a antibody that detects Hog1 in yeast species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hog1, also known as Ssk3, plays a crucial role in the osmotic stress response of yeast cells, functioning as a mitogen-activated protein kinase (MAPK) that transmits signals from the environment to the cell′s interior. Upon exposure to high external osmolarity, Hog1 is activated through a cascade involving the MAPKK Pbs2, which is essential for the regulation of osmo-adaptive responses. The activation of Hog1 leads to the modulation of transcription factors that enhance the expression of osmo-regulatory proteins, thereby enabling yeast cells to maintain their internal osmolarity and survive in fluctuating environmental conditions. Anti-Hog1 antibody (A-8) serves as a valuable tool for researchers studying stress responses and signal transduction in yeast through interactions with various upstream regulatory kinases and downstream transcription factors.

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Hog1 Antibody (A-8) References:

1. The p38 and Hog1 SAPKs control cell cycle progression in response to environmental stresses.  |  Duch, A., et al. 2012. FEBS Lett. 586: 2925-31. PMID: 22820251
2. Hog1 targets Whi5 and Msa1 transcription factors to downregulate cyclin expression upon stress.  |  González-Novo, A., et al. 2015. Mol Cell Biol. 35: 1606-18. PMID: 25733686
3. Regulation of MAP kinase Hog1 by calmodulin during hyperosmotic stress.  |  Kim, J., et al. 2016. Biochim Biophys Acta. 1863: 2551-2559. PMID: 27421986
4. Osmostress enhances activating phosphorylation of Hog1 MAP kinase by mono-phosphorylated Pbs2 MAP2K.  |  Tatebayashi, K., et al. 2020. EMBO J. 39: e103444. PMID: 32011004
5. Hog1 activation delays mitotic exit via phosphorylation of Net1.  |  Tognetti, S., et al. 2020. Proc Natl Acad Sci U S A. 117: 8924-8933. PMID: 32265285
6. The regulation of Net1/Cdc14 by the Hog1 MAPK upon osmostress unravels a new mechanism regulating mitosis.  |  Jiménez, J., et al. 2020. Cell Cycle. 19: 2105-2118. PMID: 32794416
7. RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation.  |  Guaragnella, N., et al. 2021. Microorganisms. 9: PMID: 34576788
8. HOG1 Mitogen-Activated Protein Kinase Pathway-Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia.  |  Yan, D., et al. 2023. J Fungi (Basel). 9: PMID: 37108868
9. Positive feedback induces switch between distributive and processive phosphorylation of Hog1.  |  Mosbacher, M., et al. 2023. Nat Commun. 14: 2477. PMID: 37120434
10. Role of Pmk1, Mpk1, or Hog1 in the mitogen-activated protein kinase pathway of Aspergillus cristatus.  |  Liu, L., et al. 2024. Fungal Genet Biol. 171: 103874. PMID: 38307402

The preferred Hog1 antibody is Hog1 Antibody (D-3): sc-165978.