hnRNP UL1 CRISPR/Cas9 KO Plasmid (m): sc-433226
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP UL1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hnRNP UL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP UL1 Antibody (C-2): sc-393975
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP UL1 CRISPR/Cas9 KO Plasmid (m) | sc-433226 | 20 µg | $397.00 |
Overview
Mouse Hnrnpul1 encodes hnRNP UL1, a nuclear RNA-binding protein that associates with ribonucleoprotein complexes to coordinate pre-mRNA processing, RNA transport, and broader gene expression programs. hnRNP UL1 has also been linked to DNA damage responses through interactions with repair-associated factors, supporting genome maintenance during replication stress. Through these roles, Hnrnpul1 can influence pathways governing RNA maturation, chromatin-associated transactions, and cell-cycle progression. Altered regulation of hnRNP family members is frequently investigated in contexts of genomic instability and dysregulated transcriptome control relevant to cancer biology and developmental phenotypes.
hnRNP UL1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hnrnpul1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hnrnpul1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hnrnpul1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP UL1 protein expression.
This CRISPR knockout system enables efficient generation of Hnrnpul1-deficient cell models for investigation of hnRNP UL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hnrnpul1 exon(s) critical for hnRNP UL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hnrnpul1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hnRNP UL1 CRISPR/Cas9 KO Plasmid (m) and hnRNP UL1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hnrnpul1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hnRNP UL1 HDR Plasmid (m) and hnRNP UL1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hnrnpul1 homology arms to support homology-directed repair at defined Hnrnpul1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.