hnRNP H3 Antibody (D-4): sc-376416
- hnRNP H3 Antibody (D-4) is a mouse monoclonal IgG1 κ hnRNP H3 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 155-185 within an internal region of hnRNP H3 of human origin
- hnRNP H3 Antibody (D-4) is recommended for detection of hnRNP H3 isoforms 1, 2, 3, 4, 5 and 6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-hnRNP H3 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hnRNP H3 Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with hnRNP H3 Antibody (D-4) (see ordering information below).
hnRNP H3 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP H3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP H3 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein H3, also known as hnRNP 2H9, is a crucial player in RNA processing, consisting of 346 amino acids and featuring two RNA recognition motif (RRM) domains that facilitate binding to single-stranded RNA. hnRNP H3 is particularly important in regulating pre-mRNA splicing and responding to heat shock, where hnRNP H3 plays a role in splicing arrest. Six isoforms of hnRNP H3 are generated through alternative splicing, allowing for functional diversity in RNA metabolism. hnRNP H3 predominantly resides in the nucleus, where hnRNP H3 interacts with other hnRNPs, such as hnRNP F, to bind to poly(rG) regions on RNA, thereby influencing mRNA stability and transport. The ability of hnRNP H3 to shuttle between the nucleus and cytoplasm underscores hnRNP H3′s significance in post-transcriptional regulation, making anti-hnRNP H3 antibody (D-4) an essential tool for researchers studying RNA biology and gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hnRNP H3 Antibody (D-4) References:
- The hnRNP 2H9 gene, which is involved in the splicing reaction, is a multiply spliced gene. | Honoré, B. 2000. Biochim Biophys Acta. 1492: 108-19. PMID: 10858537
- Spatiotemporal regulation of hnRNP M and 2H9 gene expression during mouse embryonic development. | Mahé, D., et al. 2000. Biochim Biophys Acta. 1492: 414-24. PMID: 11004512
- A post-translational modification of nuclear proteins, N(G),N(G)-dimethyl-Arg, found in a natural HLA class I peptide ligand. | Yagüe, J., et al. 2000. Protein Sci. 9: 2210-7. PMID: 11152131
- Nucleolar proteome dynamics. | Andersen, JS., et al. 2005. Nature. 433: 77-83. PMID: 15635413
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. | Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
- Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. Relation with splicing and early heat shock-induced splicing arrest. | Mahé, D., et al. 1997. J Biol Chem. 272: 1827-36. PMID: 8999868
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP H3 Antibody (D-4) | sc-376416 | 200 µg/ml | $322.00 | |||
hnRNP H3 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-522950 | 200 µg Ab, 40 µg BP | $361.00 | |||
hnRNP H3 Antibody (D-4): m-IgG1 BP-HRP Bundle | sc-533539 | 200 µg Ab; 20 µg BP | $361.00 | |||
hnRNP H3 Antibody (D-4) AC | sc-376416 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hnRNP H3 Antibody (D-4) HRP | sc-376416 HRP | 200 µg/ml | $322.00 | |||
hnRNP H3 Antibody (D-4) FITC | sc-376416 FITC | 200 µg/ml | $336.00 | |||
hnRNP H3 Antibody (D-4) PE | sc-376416 PE | 200 µg/ml | $349.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 488 | sc-376416 AF488 | 200 µg/ml | $364.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 546 | sc-376416 AF546 | 200 µg/ml | $364.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 594 | sc-376416 AF594 | 200 µg/ml | $364.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 647 | sc-376416 AF647 | 200 µg/ml | $364.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 680 | sc-376416 AF680 | 200 µg/ml | $364.00 | |||
hnRNP H3 Antibody (D-4) Alexa Fluor® 790 | sc-376416 AF790 | 200 µg/ml | $364.00 | |||
hnRNP H3 (D-4) Neutralizing Peptide | sc-376416 P | 100 µg/0.5 ml | $69.00 |