hnRNP F Antibody (3H4): sc-32309
- hnRNP F Antibody (3H4) is a mouse monoclonal IgG1 κ hnRNP F antibody, cited in 5 publications, provided at 200 µg/ml
- raised against full length recombinant hnRNPF of human origin
- hnRNP F Antibody (3H4) is recommended for detection of hnRNP F of human origin by WB, IP, IF and ELISA
- Anti-hnRNP F Antibody (3H4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for hnRNP F Antibody (3H4) for WB applications. These reagents are now offered in bundles with hnRNP F Antibody (3H4) (see ordering information below).
hnRNP F Antibody (3H4) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP F of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP F antibody (3H4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein F (hnRNP F) plays a crucial role in regulating pre-mRNA processing and transport, binding to heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II. hnRNP F is particularly important for involvement in the spliceosome, interacting with other splicing factors to influence splice site selection and pre-mRNA maturation. Notably, hnRNP F and its closely related counterpart, hnRNP H, preferentially associate with poly(rG) regions on RNA, essential for regulating gene expression and mRNA stability. hnRNP F shuttles between the nucleus and cytoplasm to participate in various cellular processes, including mRNA transport and localization. Additionally, hnRNP F undergoes post-translational modifications, such as phosphorylation and methylation, which can affect function and interactions with other proteins, highlighting a dynamic role in cellular regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hnRNP F Antibody (3H4) References:
- hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. | Mauger, DM., et al. 2008. Mol Cell Biol. 28: 5403-19. PMID: 18573884
- hnRNP A1 and hnRNP F modulate the alternative splicing of exon 11 of the insulin receptor gene. | Talukdar, I., et al. 2011. PLoS One. 6: e27869. PMID: 22132154
- hnRNP F complexes with tristetraprolin and stimulates ARE-mRNA decay. | Reznik, B., et al. 2014. PLoS One. 9: e100992. PMID: 24978456
- HnRNP-F regulates EMT in bladder cancer by mediating the stabilization of Snail1 mRNA by binding to its 3' UTR. | Li, F., et al. 2019. EBioMedicine. 45: 208-219. PMID: 31221586
- HnRNP-F promotes cell proliferation by regulating TPX2 in bladder cancer. | Li, F., et al. 2019. Am J Transl Res. 11: 7035-7048. PMID: 31814907
- HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. | Xu, C., et al. 2020. Cell Death Differ. 27: 1998-2013. PMID: 31863069
- HnRNP-F promotes the proliferation of bladder cancer cells mediated by PI3K/AKT/FOXO1. | Li, F., et al. 2021. J Cancer. 12: 281-291. PMID: 33391425
- HnRNP F and hnRNP H1 regulate mRNA stability of amyloid precursor protein. | Khan, MI., et al. 2021. Neuroreport. 32: 824-832. PMID: 33994531
- Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication. | Zhang, A., et al. 2022. Virol J. 19: 82. PMID: 35570267
- The RNA-binding protein hnRNP F is required for the germinal center B cell response. | Huang, H., et al. 2023. Nat Commun. 14: 1731. PMID: 36997512
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP F Antibody (3H4) | sc-32309 | 200 µg/ml | $322.00 | |||
hnRNP F Antibody (3H4): m-IgG Fc BP-HRP Bundle | sc-526677 | 200 µg Ab; 10 µg BP | $361.00 | |||
hnRNP F Antibody (3H4): m-IgG1 BP-HRP Bundle | sc-532050 | 200 µg Ab; 20 µg BP | $361.00 | |||
hnRNP F Antibody (3H4) AC | sc-32309 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hnRNP F Antibody (3H4) HRP | sc-32309 HRP | 200 µg/ml | $322.00 | |||
hnRNP F Antibody (3H4) FITC | sc-32309 FITC | 200 µg/ml | $336.00 | |||
hnRNP F Antibody (3H4) PE | sc-32309 PE | 200 µg/ml | $349.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 488 | sc-32309 AF488 | 200 µg/ml | $364.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 546 | sc-32309 AF546 | 200 µg/ml | $364.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 594 | sc-32309 AF594 | 200 µg/ml | $364.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 647 | sc-32309 AF647 | 200 µg/ml | $364.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 680 | sc-32309 AF680 | 200 µg/ml | $364.00 | |||
hnRNP F Antibody (3H4) Alexa Fluor® 790 | sc-32309 AF790 | 200 µg/ml | $364.00 |