hnRNP F/H Antibody (1G11): sc-32310
- hnRNP F/H Antibody (1G11) is a mouse monoclonal IgG1 λ hnRNP F/H antibody, cited in 18 publications, provided at 200 µg/ml
- raised against purified recombinant hnRNP F/H of human origin
- hnRNP F/H Antibody (1G11) is recommended for detection of hnRNP F and hnRNP H of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)
- Anti-hnRNP F/H Antibody (1G11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for hnRNP F/H Antibody (1G11) for WB and IHC(P) applications. This reagent is now offered in a bundle with hnRNP F/H Antibody (1G11) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
hnRNP F/H Antibody (1G11) is a mouse monoclonal IgG1 antibody that detects hnRNP F/H protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-hnRNP F/H antibody (1G11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoproteins (hnRNPs) play a crucial role in pre-mRNA processing and transport, binding to heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II. Among these, hnRNP F and H are particularly important as they preferentially associate with poly(rG) regions on RNA, influencing the regulation of gene expression. hnRNP F and H proteins are involved in the formation of hnRNP complexes, which are essential constituents of the spliceosome, and interact with other splicing factors to modulate splice site selection on pre-mRNA. hnRNP F and H undergo post-translational modifications, including phosphorylation and methylation, which can affect function and interactions with other proteins. Understanding the dynamics of hnRNP F and H, including interactions and modifications, is vital for elucidating roles in RNA metabolism and gene expression regulation, making hnRNP F/H (1G11) monoclonal antibody an invaluable research tool.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hnRNP F/H Antibody (1G11) References:
- Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs. | Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
- Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. | Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
- Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. | Martinez-Contreras, R., et al. 2006. PLoS Biol. 4: e21. PMID: 16396608
- hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. | Mauger, DM., et al. 2008. Mol Cell Biol. 28: 5403-19. PMID: 18573884
- The hnRNP F/H homologue of Trypanosoma brucei is differentially expressed in the two life cycle stages of the parasite and regulates splicing and mRNA stability. | Gupta, SK., et al. 2013. Nucleic Acids Res. 41: 6577-94. PMID: 23666624
- A cytoplasmic quaking I isoform regulates the hnRNP F/H-dependent alternative splicing pathway in myelinating glia. | Mandler, MD., et al. 2014. Nucleic Acids Res. 42: 7319-29. PMID: 24792162
- HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. | Xu, C., et al. 2020. Cell Death Differ. 27: 1998-2013. PMID: 31863069
- Isolation and characterization of a novel, low abundance hnRNP protein: A0. | Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
- A nuclear localization domain in the hnRNP A1 protein. | Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
- Three new members of the RNP protein family in Xenopus. | Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
- Identification and characterisation of a novel human RNA-binding protein. | Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
- Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. | Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP F/H Antibody (1G11) | sc-32310 | 200 µg/ml | $322.00 | |||
hnRNP F/H Antibody (1G11): m-IgGλ BP-HRP Bundle | sc-520839 | 200 µg Ab, 40 µg BP | $361.00 | |||
hnRNP F/H Antibody (1G11) AC | sc-32310 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hnRNP F/H Antibody (1G11) HRP | sc-32310 HRP | 200 µg/ml | $322.00 | |||
hnRNP F/H Antibody (1G11) FITC | sc-32310 FITC | 200 µg/ml | $336.00 | |||
hnRNP F/H Antibody (1G11) PE | sc-32310 PE | 200 µg/ml | $349.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 488 | sc-32310 AF488 | 200 µg/ml | $364.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 546 | sc-32310 AF546 | 200 µg/ml | $364.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 594 | sc-32310 AF594 | 200 µg/ml | $364.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 647 | sc-32310 AF647 | 200 µg/ml | $364.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 680 | sc-32310 AF680 | 200 µg/ml | $364.00 | |||
hnRNP F/H Antibody (1G11) Alexa Fluor® 790 | sc-32310 AF790 | 200 µg/ml | $364.00 |