Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP E1 CRISPR/Cas9 KO Plasmid (m): sc-423930
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP E1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hnRNP E1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP E1 Antibody (E-2): sc-137249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP E1 CRISPR/Cas9 KO Plasmid (m) | sc-423930 | 20 µg | $397.00 |
Overview
Pcbp1 encodes the mouse heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1), an RNA-binding protein that recognizes C-rich elements to control mRNA stability and translation. hnRNP E1 participates in post-transcriptional gene regulation linked to cell-cycle progression, stress responses, and differentiation by shaping the availability of transcripts in the cytoplasm and their translation at ribosomes. Through its roles in RNA processing and translational control, hnRNP E1 influences pathways associated with epithelial–mesenchymal transition, cytoskeletal remodeling, and signaling-dependent gene expression programs. Dysregulated PCBP1/hnRNP E1 activity has been associated with altered RNA homeostasis and phenotypes relevant to tumor biology and inflammatory signaling in experimental models.
hnRNP E1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pcbp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pcbp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pcbp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP E1 protein expression.
This CRISPR knockout system enables efficient generation of Pcbp1-deficient cell models for investigation of hnRNP E1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pcbp1 exon(s) critical for hnRNP E1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pcbp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hnRNP E1 CRISPR/Cas9 KO Plasmid (m) and hnRNP E1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pcbp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hnRNP E1 HDR Plasmid (m) and hnRNP E1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pcbp1 homology arms to support homology-directed repair at defined Pcbp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.