hnRNP C1/C2 Antibody (B-8): sc-515938

hnRNP C1/C2 Antibody (B-8) is a mouse monoclonal IgG1 antibody that detects hnRNP C1/C2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). hnRNP C1 and C2 are essential components of the heterogeneous nuclear ribonucleoprotein complexes, playing a critical role in the processing and transport of pre-mRNA. These proteins are primarily located in the nucleus, where they bind to heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II, facilitating the assembly of spliceosomal complexes necessary for mRNA maturation. The ability of hnRNP C1 and C2 to shuttle between the nucleus and cytoplasm is vital for regulating gene expression, as this movement allows for dynamic response to cellular signals and modulation of mRNA stability and translation. Additionally, hnRNP C1 and C2 contain multiple RNA binding domains, which enable interaction with various RNA sequences, influencing splicing decisions and splice site selection. Post-translational modifications, such as phosphorylation and methylation, further diversify the functional repertoire of hnRNP C1 and C2, allowing for fine-tuned regulation of gene expression in response to cellular conditions. Anti-hnRNP C1/C2 antibody (B-8) serves as an invaluable tool for researchers studying RNA processing and gene regulation mechanisms in diverse biological contexts.

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hnRNP C1/C2 Antibody (B-8) References:

1. A 57-nucleotide upstream early polyadenylation element in human papillomavirus type 16 interacts with hFip1, CstF-64, hnRNP C1/C2, and polypyrimidine tract binding protein.  |  Zhao, X., et al. 2005. J Virol. 79: 4270-88. PMID: 15767428
2. Downregulation of hnRNP C1/C2 by siRNA sensitizes HeLa cells to various stresses.  |  Hossain, MN., et al. 2007. Mol Cell Biochem. 296: 151-7. PMID: 16960656
3. Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein.  |  Noisakran, S., et al. 2008. Biochem Biophys Res Commun. 372: 67-72. PMID: 18471994
4. HnRNP C1/C2 may regulate exon 7 splicing in the spinal muscular atrophy gene SMN1.  |  Irimura, S., et al. 2009. Kobe J Med Sci. 54: E227-36. PMID: 19628962
5. Altering the expression balance of hnRNP C1 and C2 changes the expression of myelination-related genes.  |  Iwata, K., et al. 2011. Psychiatry Res. 190: 364-6. PMID: 21684615
6. Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2.  |  Huang, L., et al. 2011. Mol Cell Biol. 31: 3472-84. PMID: 21690294
7. The Ebola virus VP24 protein prevents hnRNP C1/C2 binding to karyopherin α1 and partially alters its nuclear import.  |  Shabman, RS., et al. 2011. J Infect Dis. 204 Suppl 3: S904-10. PMID: 21987768
8. Strand-specific affinity of host factor hnRNP C1/C2 guides positive to negative-strand ratio in Coxsackievirus B3 infection.  |  Dave, P., et al. 2019. RNA Biol. 16: 1286-1299. PMID: 31234696
9. Variant in NHLRC2 leads to increased hnRNP C2 in developing neurons and the hippocampus of a mouse model of FINCA disease.  |  Hiltunen, AE., et al. 2020. Mol Med. 26: 123. PMID: 33297935
10. Inhibition of the apolipoprotein B mRNA editing enzyme-complex by hnRNP C1 protein and 40S hnRNP complexes.  |  Greeve, J., et al. 1998. Biol Chem. 379: 1063-73. PMID: 9792439

The preferred hnRNP C1/C2 antibody is hnRNP C1/C2 Antibody (4F4): sc-32308.