hnRNP A1 Antibody (4B10): sc-32301
- hnRNP A1 Antibody (4B10) is a mouse monoclonal IgG2a κ hnRNP A1 antibody, cited in 149 publications, provided at 200 µg/ml
- raised against full length partially purified hnRNP A1
- hnRNP A1 Antibody (4B10) is recommended for detection of hnRNP A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and canine
- Anti-hnRNP A1 Antibody (4B10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hnRNP A1 Antibody (4B10) for WB and IHC(P) applications. These reagents are now offered in bundles with hnRNP A1 Antibody (4B10) (see ordering information below).
hnRNP A1 Antibody (4B10) is a mouse monoclonal IgG2a kappa light chain antibody that detects hnRNP A1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP A1 antibody (4B10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) plays a crucial role in regulating pre-mRNA processing and is essential for mRNA stability and transport. Located primarily in the nucleus, hnRNP A1 interacts with pre-mRNA and influences splice site selection, thereby modulating gene expression. hnRNP A1 forms complexes with other hnRNP proteins and splicing factors, which are vital for spliceosome assembly, the molecular machinery responsible for splicing introns from pre-mRNA. hnRNP A1′s ability to shuttle between the nucleus and cytoplasm enables participation in various stages of mRNA metabolism, making hnRNP A1 a key player in post-transcriptional regulation. hnRNP A1′s involvement in mRNA transport and stability underscores its importance in maintaining proper gene expression and cellular function, particularly in response to stress and during development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hnRNP A1 Antibody (4B10) References:
- Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs. | Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
- Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. | Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
- hnRNP A1 in RNA metabolism regulation and as a potential therapeutic target. | Feng, J., et al. 2022. Front Pharmacol. 13: 986409. PMID: 36339596
- Retraction Note: HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395. | Sun, G., et al. 2023. J Exp Clin Cancer Res. 42: 43. PMID: 36755337
- The microRNA-195-5p/hnRNP A1 axis contributes to the progression of hepatocellular carcinoma by regulating the migration of cancer cells. | Jung, M., et al. 2023. Biochem Biophys Res Commun. 686: 149183. PMID: 37926044
- hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS). | Salapa, HE., et al. 2024. Nat Commun. 15: 356. PMID: 38191621
- hnRNP A1, hnRNP A2B1, and hnRNP K are dysregulated in tauopathies, but do not colocalize with tau pathology. | Kavanagh, T., et al. 2024. Brain Pathol. e13305. PMID: 39354671
- Isolation and characterization of a novel, low abundance hnRNP protein: A0. | Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
- A nuclear localization domain in the hnRNP A1 protein. | Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
- Three new members of the RNP protein family in Xenopus. | Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
- Identification and characterisation of a novel human RNA-binding protein. | Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
- Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. | Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A1 Antibody (4B10) | sc-32301 | 200 µg/ml | $322.00 | |||
hnRNP A1 Antibody (4B10): m-IgG Fc BP-HRP Bundle | sc-528456 | 200 µg Ab; 10 µg BP | $361.00 | |||
hnRNP A1 Antibody (4B10): m-IgGκ BP-HRP Bundle | sc-520837 | 200 µg Ab, 40 µg BP | $361.00 | |||
hnRNP A1 Antibody (4B10) AC | sc-32301 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hnRNP A1 Antibody (4B10) HRP | sc-32301 HRP | 200 µg/ml | $322.00 | |||
hnRNP A1 Antibody (4B10) FITC | sc-32301 FITC | 200 µg/ml | $336.00 | |||
hnRNP A1 Antibody (4B10) PE | sc-32301 PE | 200 µg/ml | $349.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 488 | sc-32301 AF488 | 200 µg/ml | $364.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 546 | sc-32301 AF546 | 200 µg/ml | $364.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 594 | sc-32301 AF594 | 200 µg/ml | $364.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 647 | sc-32301 AF647 | 200 µg/ml | $364.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 680 | sc-32301 AF680 | 200 µg/ml | $364.00 | |||
hnRNP A1 Antibody (4B10) Alexa Fluor® 790 | sc-32301 AF790 | 200 µg/ml | $364.00 |