hnRNP A1 Antibody (4B10): sc-32301

hnRNP A1 Antibody (4B10) is a mouse monoclonal IgG2a kappa light chain antibody that detects hnRNP A1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP A1 antibody (4B10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) plays a crucial role in regulating pre-mRNA processing and is essential for mRNA stability and transport. Located primarily in the nucleus, hnRNP A1 interacts with pre-mRNA and influences splice site selection, thereby modulating gene expression. hnRNP A1 forms complexes with other hnRNP proteins and splicing factors, which are vital for spliceosome assembly, the molecular machinery responsible for splicing introns from pre-mRNA. hnRNP A1′s ability to shuttle between the nucleus and cytoplasm enables participation in various stages of mRNA metabolism, making hnRNP A1 a key player in post-transcriptional regulation. hnRNP A1′s involvement in mRNA transport and stability underscores its importance in maintaining proper gene expression and cellular function, particularly in response to stress and during development.

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hnRNP A1 Antibody (4B10) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
3. hnRNP A1 in RNA metabolism regulation and as a potential therapeutic target.  |  Feng, J., et al. 2022. Front Pharmacol. 13: 986409. PMID: 36339596
4. Retraction Note: HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.  |  Sun, G., et al. 2023. J Exp Clin Cancer Res. 42: 43. PMID: 36755337
5. The microRNA-195-5p/hnRNP A1 axis contributes to the progression of hepatocellular carcinoma by regulating the migration of cancer cells.  |  Jung, M., et al. 2023. Biochem Biophys Res Commun. 686: 149183. PMID: 37926044
6. hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS).  |  Salapa, HE., et al. 2024. Nat Commun. 15: 356. PMID: 38191621
7. hnRNP A1, hnRNP A2B1, and hnRNP K are dysregulated in tauopathies, but do not colocalize with tau pathology.  |  Kavanagh, T., et al. 2024. Brain Pathol. e13305. PMID: 39354671
8. Isolation and characterization of a novel, low abundance hnRNP protein: A0.  |  Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
9. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
10. Three new members of the RNP protein family in Xenopus.  |  Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
11. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
12. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors.  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP A1 Antibody (4B10) has 149 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP A1 Antibody (4B10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP A1 Antibody (4B10) Specifications

Alternate Product Name: hnRNP A1

Product Clone Name: 4B10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: hnRNP A1

Epitope Species: human

Product Reactivity/Detection: hnRNP A1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 149 citations in various scientific publications

Available Conjugates/Forms for hnRNP A1 Antibody (4B10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin