HNF-3γ Antibody (A-2): sc-74424

HNF-3γ Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects HNF-3 gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HNF-3γ (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HNF-3 gamma protein plays a crucial role in regulating gene expression in the liver and other tissues, and is part of the hepatocyte nuclear factor family, which includes HNF-1 (α and β), HNF-3 (α, β, and γ), HNF-4 (α and γ), and HNF-6. These proteins are essential for transcriptional regulation of genes involved in metabolic processes and organ development. Specifically, HNF-3γ interacts with other transcription factors, such as HNF-1 and HNF-4, to coordinate expression of genes critical for liver function and development. Proper functioning of HNF-3γ is vital, as mutations or dysregulation can lead to metabolic disorders and developmental abnormalities. Understanding interactions and regulatory mechanisms involving HNF-3γ is important for elucidating its role in health and disease, making anti-HNF-3γ antibody (A-2) a valuable tool for researchers studying these processes.

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HNF-3γ Antibody (A-2) References:

1. Hepatoprotective action of adenovirus-transferred HNF-3gamma gene in acute liver injury caused by CCl(4).  |  Nakamura, T., et al. 1999. FEBS Lett. 459: 1-4. PMID: 10508906
2. Foxa3 (HNF-3gamma) binds to and activates the rat proglucagon gene promoter but is not essential for proglucagon gene expression.  |  Liu, Y., et al. 2002. Biochem J. 366: 633-41. PMID: 12000309
3. Role of hepatocyte nuclear factors in transcriptional regulation of male-specific CYP2A2.  |  Wiwi, CA. and Waxman, DJ. 2005. J Biol Chem. 280: 3259-68. PMID: 15539409
4. Growth hormone regulates the expression of hepatocyte nuclear factor-3 gamma and other liver-enriched transcription factors in the bovine liver.  |  Eleswarapu, S. and Jiang, H. 2005. J Endocrinol. 184: 95-105. PMID: 15642787
5. FOXA3 induction under endoplasmic reticulum stress contributes to non-alcoholic fatty liver disease.  |  Liu, C., et al. 2021. J Hepatol. 75: 150-162. PMID: 33548387
6. Direct reprogramming of fibroblasts into functional hepatocytes via CRISPRa activation of endogenous Gata4 and Foxa3.  |  Li, J., et al. 2024. Chin Med J (Engl). 137: 1351-1359. PMID: 38721807
7. FOXA3 regulates cholesterol metabolism to compensate for low uptake during the progression of lung adenocarcinoma.  |  Wang, D., et al. 2024. PLoS Biol. 22: e3002621. PMID: 38805565
8. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing.  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
9. The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution.  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
10. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
11. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression.  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657
12. Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats.  |  Nakamura, T., et al. 1998. Biochem Biophys Res Commun. 253: 352-7. PMID: 9878541

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-3γ Antibody (A-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-3γ Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-3γ Antibody (A-2) Specifications

Alternate Product Name: FOXA3

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-115

Epitope Target: HNF-3Γ

Epitope Species: human

Product Reactivity/Detection: HNF-3γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HNF-3γ Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent