HNF-3 gamma Antibody (A-2) recommended for detection of HNF-3γ of mouse, rat and human origin by WB, IP, IF and ELISA

HNF-3γ Antibody (A-2): sc-74424

HNF-3γ Antibody (A-2) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-HNF-3γ Antibody (A-2) is a mouse monoclonal IgG1 (kappa light chain) HNF-3γ antibody provided at 200 µg/ml
    • raised against amino acids 1-115 of HNF-3Γ of human origin
    • Anti-HNF-3 gamma Antibody (A-2) is recommended for detection of HNF-3γ of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HNF-3 gamma Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-74424 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-3γ (A-2): sc-74424.
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What’s the concentration of protein Lysate loaded for the hepg2 wb and the dilution of primary ab used? Thanks

Asked by: Kp233
Thank you for the question. We suggest to follow our WB protocol https://www.scbt.com/scbt/de/resources/protocols/western-immuno-blotting and prior to optimization start with the antibody concentration at 1:200 and load 50µg protein HepG2 lysate.
Answered by: Technical Support Europe
Date published: 2018-02-05

When should the TransCruz reagent sc-74424 X be used instead of the standard form sc-74424?

Asked by: Randy McDonald
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Specific and strong band detected in Hep Specific and strong band detected in Hep G2 whole cell lysate and Hep G2 nuclear extract. - SCBT QC
Date published: 2015-02-19
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