HNF-3β Antibody (A-12): sc-374375
- HNF-3β Antibody (A-12) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 431-459 at the C-terminus of HNF-3β of mouse origin
- Anti-HNF-3 beta Antibody (A-12) is recommended for detection of HNF-3β of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- TransCruz reagent for ChIP application (sc-374375 X, 200 µg/0.1 ml)
- Anti-HNF-3 beta Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for HNF-3β Antibody (A-12) for WB applications. This reagent is now offered in a bundle with HNF-3β Antibody (A-12) (see ordering information below).
HNF-3β Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 431-459 at the C-terminus of HNF-3β of mouse origin, exhibiting reactivity with mouse, rat, and human proteins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), and also reactive with additional species including equine, canine, bovine, and porcine. HNF-3β, also known as FOXA2 (Forkhead Box A2), is a critical transcription factor with a conserved forkhead DNA-binding domain that regulates genes essential for liver development, metabolism, and differentiation of endoderm-derived tissues. The function of HNF-3β is modulated by post-translational modifications such as phosphorylation, acetylation, and sumoylation, which influence transcriptional activity, stability, nuclear localization, and DNA-binding affinity. For instance, phosphorylation can alter HNF-3β′s interaction with other proteins and DNA, affecting gene expression patterns during development and in response to cellular signaling pathways. The hepatocyte nuclear factor family, comprising HNF-1 (α and β), HNF-3 (α, β, and γ), HNF-4 (α and γ), and HNF-6, regulates transcription of genes in the liver and other tissues like the kidney, small intestine, and thymus, with HNF-3α, HNF-3β, and HNF-3γ involved in controlling numerous hepatocyte genes in the adult liver and playing roles in gastrulation events such as body axis formation. HNF-6 binds to the promoter of HNF-3β, indicating a potential role in gut endoderm epithelial cell differentiation, and may act as a transcriptional activator for multiple hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin, making anti-HNF-3β antibody (A-12) a valuable tool for researchers studying the role of HNF-3β and post-translational modifications in various biological processes and diseases such as diabetes, liver dysfunction, and cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNF-3β Antibody (A-12) | sc-374375 | 200 µg/ml | $316.00 | |||
HNF-3β Antibody (A-12): m-IgG Fc BP-HRP Bundle | sc-525959 | 200 µg Ab; 10 µg BP | $354.00 | |||
HNF-3β Antibody (A-12) X | sc-374375 X | 200 µg/0.1 ml | $316.00 | |||
HNF-3β Antibody (A-12) AC | sc-374375 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HNF-3β Antibody (A-12) HRP | sc-374375 HRP | 200 µg/ml | $316.00 | |||
HNF-3β Antibody (A-12) FITC | sc-374375 FITC | 200 µg/ml | $330.00 | |||
HNF-3β Antibody (A-12) PE | sc-374375 PE | 200 µg/ml | $343.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 488 | sc-374375 AF488 | 200 µg/ml | $357.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 546 | sc-374375 AF546 | 200 µg/ml | $357.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 594 | sc-374375 AF594 | 200 µg/ml | $357.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 647 | sc-374375 AF647 | 200 µg/ml | $357.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 680 | sc-374375 AF680 | 200 µg/ml | $357.00 | |||
HNF-3β Antibody (A-12) Alexa Fluor® 790 | sc-374375 AF790 | 200 µg/ml | $357.00 | |||
HNF-3β (A-12) Neutralizing Peptide | sc-374375 P | 100 µg/0.5 ml | $68.00 |