HNF-1 alpha Antibody (2) recommended for detection of HNF-1α of mouse, rat and human origin by WB, IP and IF

HNF-1α Antibody (2): sc-135939

HNF-1α Antibody (2) is rated 4.0 out of 5 by 3.
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5
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4
3
3
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2
0
1
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Datasheets

    • HNF-1α Antibody (2) is a mouse monoclonal IgG1 provided at 200 µg/ml
    • raised against amino acids 70-269 of HNF-1α of mouse origin
    • recommended for detection of HNF-1α of mouse, rat and human origin by WB, IP and IF
    • See HNF-1α (F-7): sc-393925 for HNF-1α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using HNF-1α (2): sc-135939 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody Works as we expected. Tested by immunofluorescence.
Date published: 2018-12-18
Rated 4 out of 5 by from Clean band seen in WB with rat liver tissue Clean band seen in WB with rat liver tissue extract. -SCBT QC
Date published: 2015-02-21
Rated 4 out of 5 by from Good nuclear staining of endothelial cells Good nuclear staining of endothelial cells seen in IF -SCBT QC
Date published: 2013-04-28
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