HMGCR Antibody (C-1): sc-271595

HMGCR Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HMGCR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HMGCR antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The human enzyme hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) plays a critical role in regulating cholesterol synthesis, which is essential for cellular growth and membrane integrity, particularly in liver tissue. HMGCR activity is tightly controlled through phosphorylation, a post-translational modification that inactivates HMGCR in response to sterols and non-sterol metabolites, thereby preventing excessive cholesterol production. This regulatory mechanism is vital for maintaining lipid homeostasis and preventing conditions such as hypercholesterolemia. HMGCR serves as the target of statins, which are widely used to lower plasma cholesterol levels and reduce the risk of cardiovascular events, highlighting HMGCR′s importance in both metabolic regulation and therapeutic interventions. Intermediates produced in the cholesterol synthesis pathway can influence signaling pathways, such as those involving Ras, suggesting that HMGCR regulation may have implications for novel chemotherapeutic strategies.

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HMGCR Antibody (C-1) References:

1. The mevalonate pathway during acute tubular injury: selected determinants and consequences.  |  Zager, RA., et al. 2002. Am J Pathol. 161: 681-92. PMID: 12163393
2. Molecular regulation of cholesterol biosynthesis: implications in carcinogenesis.  |  Singh, RP., et al. 2003. J Environ Pathol Toxicol Oncol. 22: 75-92. PMID: 14533871
3. Rapid effect of 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibition on coronary endothelial function.  |  Wassmann, S., et al. 2003. Circ Res. 93: e98-103. PMID: 14551237
4. HMG-CoA reductase inhibition reduces the proinflammatory activation of human vascular smooth muscle cells by the terminal complement factor C5b-9.  |  Viedt, C., et al. 2003. Basic Res Cardiol. 98: 353-61. PMID: 14556080
5. Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved domains responsible for catalytic activity and sterol-regulated degradation.  |  Luskey, KL. and Stevens, B. 1985. J Biol Chem. 260: 10271-7. PMID: 2991281
6. Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.  |  Lu, XY., et al. 2020. Nature. 588: 479-484. PMID: 33177714
7. AMPK promotes antitumor immunity by downregulating PD-1 in regulatory T cells via the HMGCR/p38 signaling pathway.  |  Pokhrel, RH., et al. 2021. Mol Cancer. 20: 133. PMID: 34649584
8. Limb girdle muscular disease caused by HMGCR mutation and statin myopathy treatable with mevalonolactone.  |  Yogev, Y., et al. 2023. Proc Natl Acad Sci U S A. 120: e2217831120. PMID: 36745799
9. Association of HMGCR inhibition with rheumatoid arthritis: a Mendelian randomization and colocalization study.  |  Ma, L., et al. 2023. Front Endocrinol (Lausanne). 14: 1272167. PMID: 38047111
10. BRCC36 Deubiquitinates HMGCR to Regulate the Interplay Between Ferroptosis and Pyroptosis.  |  Wang, H., et al. 2024. Adv Sci (Weinh). 11: e2304263. PMID: 38178583
11. Inhibiting HMGCR represses stemness and metastasis of hepatocellular carcinoma via Hedgehog signaling.  |  Zhang, Z., et al. 2024. Genes Dis. 11: 101285. PMID: 39022130
12. Cellular expression of an HMGCR promoter-CAT fusion gene in transgenic mouse brain: evidence for a developmental regulation in oligodendrocytes.  |  Duhamel-Clerin, E., et al. 1994. Glia. 11: 35-46. PMID: 8070893

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HMGCR Antibody (C-1) has 65 citations in a variety of scientific publications.

Primary antibodies like Anti-HMGCR Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HMGCR Antibody (C-1) Specifications

Alternate Product Name: HMGCR

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 589-888

Epitope Target: HMGCR

Epitope Species: human

Product Reactivity/Detection: HMGCR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 65 citations in various scientific publications

Available Conjugates/Forms for HMGCR Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin