HMG-2/HMGB2 Antibody (D-3): sc-271689

HMG-2 Antibody (D-3) is a mouse monoclonal IgG3 antibody that detects HMG-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HMG-2 antibody (D-3) is available as the non-conjugated form. The high mobility group (HMG) proteins, including HMG-2, play a crucial role in regulating gene expression by influencing chromatin structure. HMG-2 binds to DNA and induces significant conformational changes, such as bending and unwinding of the DNA helix, which are essential for transcription factor accessibility to target genes. This dynamic interaction enables proper functioning of various cellular processes, including transcriptional activation, where HMG-2 collaborates with other transcription factors like Oct-2 and members of the NFκB family. Post-translational modifications, such as phosphorylation, enhance HMG-2 transcriptional activity, highlighting their importance in cellular signaling pathways. With unique binding capabilities to single-stranded DNA and ability to alter double-stranded DNA conformation, HMG-2 serves as a key player in chromatin remodeling and gene regulation, making anti-HMG-2 antibody (D-3) an invaluable research tool for studying these fundamental biological processes.

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HMG-2/HMGB2 Antibody (D-3) References:

1. HMG box A in HMG2 protein functions as a mediator of DNA structural alteration together with box B.  |  Nakamura, Y., et al. 2001. J Biochem. 129: 643-51. PMID: 11275566
2. HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A.  |  Fan, Z., et al. 2002. Mol Cell Biol. 22: 2810-20. PMID: 11909973
3. Structure of a gene coding for human HMG2 protein.  |  Shirakawa, H. and Yoshida, M. 1992. J Biol Chem. 267: 6641-5. PMID: 1551873
4. Structural features of the HMG chromosomal proteins and their genes.  |  Bustin, M., et al. 1990. Biochim Biophys Acta. 1049: 231-43. PMID: 2200521
5. A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.  |  Wen, L., et al. 1989. Nucleic Acids Res. 17: 1197-214. PMID: 2922262
6. HMGI(Y) and Sp1 in addition to NF-kappa B regulate transcription of the MGSA/GRO alpha gene.  |  Wood, LD., et al. 1995. Nucleic Acids Res. 23: 4210-9. PMID: 7479086
7. Interleukin 4-inducible phosphorylation of HMG-I(Y) is inhibited by rapamycin.  |  Wang, DZ., et al. 1995. J Biol Chem. 270: 22924-32. PMID: 7559428
8. Structural basis for DNA bending by the architectural transcription factor LEF-1.  |  Love, JJ., et al. 1995. Nature. 376: 791-5. PMID: 7651541
9. Existence of a transcription factor for the human HMG2 gene positively related to the level of HMG2 mRNA in the cells.  |  Shirakawa, H. and Yoshida, M. 1995. Biochemistry. 34: 2521-7. PMID: 7873532
10. Changes in superhelicity are introduced into closed circular DNA by binding of high mobility group protein I/Y.  |  Nissen, MS. and Reeves, R. 1995. J Biol Chem. 270: 4355-60. PMID: 7876198
11. Reversal of intrinsic DNA bends in the IFN beta gene enhancer by transcription factors and the architectural protein HMG I(Y).  |  Falvo, JV., et al. 1995. Cell. 83: 1101-11. PMID: 8548798
12. Prevalence and characterization of novel pANCA, antibodies to the high mobility group non-histone chromosomal proteins HMG1 and HMG2, in systemic rheumatic diseases.  |  Uesugi, H., et al. 1998. J Rheumatol. 25: 703-9. PMID: 9558172

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HMG-2/HMGB2 Antibody (D-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HMG-2/HMGB2 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HMG-2/HMGB2 Antibody (D-3) Specifications

Alternate Product Name: HMGB2

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids: 163-193

Epitope Target: HMG-2

Epitope Species: human

Product Reactivity/Detection: HMG-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HMG-2/HMGB2 Antibody (D-3): P: Blocking Peptide, X: TransCruz Reagent