HMG-2/HMGB2 Antibody (68.32): sc-101067
- HMG-2/HMGB2 Antibody (68.32) is a mouse monoclonal IgG2a κ provided at 100 µg/ml
- raised against recombinant HMG-2 of human origin
- recommended for detection of HMG-2 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HMG-2/HMGB2 Antibody (68.32). This work is in progress.
HMG-2 Antibody (68.32) is a mouse monoclonal IgG2a antibody that detects HMG-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). HMG-2, a member of the high mobility group (HMG) protein family, plays a crucial role in the regulation of gene expression by binding to DNA and inducing structural changes that facilitate transcription. HMG-2 is primarily located in the nucleus, where anti-HMG-2 antibody (68.32) can detect interactions with chromatin and influences on DNA architecture, promoting essential processes such as transcriptional activation. The ability of HMG-2 to bend and unwind DNA is vital for the recruitment of transcription factors and the assembly of the transcriptional machinery, thereby impacting cellular responses to various stimuli. Additionally, HMG-2 undergoes post-translational modifications, including phosphorylation, which can enhance transcriptional activity and further regulate gene expression. With a significant role in chromatin dynamics and gene regulation, HMG-2 monoclonal antibody (68.32) serves as an invaluable tool for researchers studying gene expression and chromatin structure in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HMG-2/HMGB2 Antibody (68.32) References:
- High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis. | Sobajima, J., et al. 1999. Gut. 44: 867-73. PMID: 10323891
- Autoantibodies to nonhistone chromosomal proteins HMG-1 and HMG-2 in sera of patients with juvenile rheumatoid arthritis. | Wittemann, B., et al. 1990. Arthritis Rheum. 33: 1378-83. PMID: 2403401
- mRNA of chromosomal proteins HMG-1 and HMG-2 are polyadenylated. | Bustin, M., et al. 1981. Biochem Biophys Res Commun. 101: 893-7. PMID: 6118142
- Comparative studies on microinjected high-mobility-group chromosomal proteins, HMG1 and HMG2. | Wu, L., et al. 1981. J Cell Biol. 91: 488-96. PMID: 6458621
- Differential expression of nuclear HMG1, HMG2 proteins and H1(zero) histone in various blood cells. | Cabart, P., et al. 1995. Cell Biochem Funct. 13: 125-33. PMID: 7758147
- Repression of basal transcription by HMG2 is counteracted by TFIIH-associated factors in an ATP-dependent process. | Stelzer, G., et al. 1994. Mol Cell Biol. 14: 4712-21. PMID: 8007973
- Identification of cDNA clones encoding HMG 2, a major protein of the mexican axolotl hydrocortisone-sensitive thymocytes. | de Guerra, A., et al. 1995. Dev Comp Immunol. 19: 417-23. PMID: 8654668
- Differential binding of HMG1, HMG2, and a single HMG box to cisplatin-damaged DNA. | Farid, RS., et al. 1996. Toxicol Appl Pharmacol. 141: 532-9. PMID: 8975778
- Nuclear accumulation of HMG2 protein is mediated by basic regions interspaced with a long DNA-binding sequence, and retention within the nucleus requires the acidic carboxyl terminus. | Shirakawa, H., et al. 1997. Biochemistry. 36: 5992-9. PMID: 9166769
- Differences in DNA recognition and conformational change activity between boxes A and B in HMG2 protein. | Yoshioka, Ki., et al. 1999. Biochemistry. 38: 589-95. PMID: 9888798
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-2/HMGB2 Antibody (68.32) | sc-101067 | 100 µg/ml | $339.00 |