HLF Antibody (4D8): sc-134359

HLF Antibody (4D8) is a mouse monoclonal IgG2b kappa light chain antibody that detects HLF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-HLF antibody (4D8) is available as the non-conjugated form. HLF protein, or hepatic leukemia factor, is a 295 amino acid nuclear protein predominantly expressed in the liver, with lower expression levels found in the lung and kidney. As a member of the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins, HLF (4D8) antibody recognizes a crucial factor in gene regulation that binds to DNA as either a homodimer or a heterodimer with other PAR factors. HLF protein′s ability to form dimers is essential for function, allowing precise regulation of target genes involved in various cellular processes, including cell survival and differentiation. Notably, chromosomal translocations that fuse portions of the HLF gene with the E2A gene are implicated in a subset of childhood B-lineage acute lymphoid leukemias, highlighting HLF′s importance in oncogenic processes. The E2A-HLF chimeric fusion protein acts as a potent trans-activator, activating SLUG, a mammalian homologue of the cell death specification protein ces-1 in Caenorhabditis elegans, which regulates an evolutionarily conserved cell survival program. HLF is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes more than 1,200 genes, underscoring its significance in both normal physiology and disease states.

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HLF Antibody (4D8) References:

1. E2A-HLF usurps control of evolutionarily conserved survival pathways.  |  Seidel, MG. and Look, AT. 2001. Oncogene. 20: 5718-25. PMID: 11607821
2. E2A basic helix-loop-helix transcription factors in human leukemia.  |  LeBrun, DP. 2003. Front Biosci. 8: s206-22. PMID: 12700034
3. Regulation of annexin II by cytokine-initiated signaling pathways and E2A-HLF oncoprotein.  |  Matsunaga, T., et al. 2004. Blood. 103: 3185-91. PMID: 15070701
4. Hlf, a novel hepatic bZIP protein, shows altered DNA-binding properties following fusion to E2A in t(17;19) acute lymphoblastic leukemia.  |  Hunger, SP., et al. 1992. Genes Dev. 6: 1608-20. PMID: 1516826
5. The loss of circadian PAR bZip transcription factors results in epilepsy.  |  Gachon, F., et al. 2004. Genes Dev. 18: 1397-412. PMID: 15175240
6. ABCB1 over-expression and drug-efflux in acute lymphoblastic leukemia cell lines with t(17;19) and E2A-HLF expression.  |  Baudis, M., et al. 2006. Pediatr Blood Cancer. 47: 757-64. PMID: 16206189
7. The rat hepatic leukemia factor (HLF) gene encodes two transcriptional activators with distinct circadian rhythms, tissue distributions and target preferences.  |  Falvey, E., et al. 1995. EMBO J. 14: 4307-17. PMID: 7556072
8. DNA-binding specificity and trans-activating potential of the leukemia-associated E2A-hepatic leukemia factor fusion protein.  |  Inaba, T., et al. 1994. Mol Cell Biol. 14: 3403-13. PMID: 8164688
9. The proto-oncogene HLF and the related basic leucine zipper protein TEF display highly similar DNA-binding and transcriptional regulatory properties.  |  Hunger, SP., et al. 1996. Blood. 87: 4607-17. PMID: 8639829

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HLF Antibody (4D8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HLF Antibody (4D8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HLF Antibody (4D8) Specifications

Alternate Product Name: HLF

Product Clone Name: 4D8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-296

Epitope Target: HLF

Epitope Species: human

Product Reactivity/Detection: HLF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HLF Antibody (4D8): N/A