HLA-DQ3 Antibody (2HB6): sc-53310
- HLA-DQ3 Antibody (2HB6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against Priess cells (an EBV-transformed B cell line) of human origin
- HLA-DQ3 Antibody (2HB6) is recommended for detection of HLA-DQ3 of mouse origin by IP and FCM
- Anti-HLA-DQ3 Antibody (2HB6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HLA-DQ3 Antibody (2HB6). These reagents are now offered in bundles with HLA-DQ3 Antibody (2HB6) (see ordering information below).
HLA-DQ3 Antibody (2HB6) is a mouse monoclonal IgG1 kappa light chain antibody that detects HLA-DQ3 of mouse origin by immunoprecipitation (IP) and flow cytometry (FCM). Anti-HLA-DQ3 antibody (2HB6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DQ3 plays a crucial role in the immune system as a major histocompatibility complex (MHC) class II molecule, which is essential for presenting peptide antigens to CD4+ T cells. This process is vital for initiating adaptive immune responses, as CD4+ T cells recognize and respond to pathogens. HLA-DQ3′s function depends on interactions with other proteins, such as HLA-DM and HLA-DO, which facilitate peptide antigen exchange by regulating the dissociation of class II-associated invariant chain peptides (CLIP). These interactions maintain the immune system′s ability to distinguish between self and non-self, thereby preventing autoimmune responses. RFLP analysis of HLA-DM genes has indicated that certain polymorphisms may contribute to susceptibility to conditions like rheumatoid arthritis, highlighting HLA-DQ3′s importance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DQ3 Antibody (2HB6) References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism. | Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DQ3 Antibody (2HB6) | sc-53310 | 200 µg/ml | $316.00 | |||
HLA-DQ3 Antibody (2HB6): m-IgG Fc BP-HRP Bundle | sc-526793 | 200 µg Ab; 10 µg BP | $354.00 | |||
HLA-DQ3 Antibody (2HB6): m-IgG1 BP-HRP Bundle | sc-532166 | 200 µg Ab; 20 µg BP | $354.00 | |||
HLA-DQ3 Antibody (2HB6) AC | sc-53310 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HLA-DQ3 Antibody (2HB6) HRP | sc-53310 HRP | 200 µg/ml | $316.00 | |||
HLA-DQ3 Antibody (2HB6) FITC | sc-53310 FITC | 200 µg/ml | $330.00 | |||
HLA-DQ3 Antibody (2HB6) PE | sc-53310 PE | 200 µg/ml | $343.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 488 | sc-53310 AF488 | 200 µg/ml | $357.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 546 | sc-53310 AF546 | 200 µg/ml | $357.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 594 | sc-53310 AF594 | 200 µg/ml | $357.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 647 | sc-53310 AF647 | 200 µg/ml | $357.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 680 | sc-53310 AF680 | 200 µg/ml | $357.00 | |||
HLA-DQ3 Antibody (2HB6) Alexa Fluor® 790 | sc-53310 AF790 | 200 µg/ml | $357.00 |