HLA-DOβ Antibody (DOB.L1): sc-69739
- HLA-DOβ Antibody (DOB.L1) is a mouse monoclonal IgG2b κ HLA-DOβ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against the intracytoplasmic portion of HLA-DO β-chain of human origin
- HLA-DO beta Antibody (DOB.L1) is recommended for detection of HLA-DOβ of human origin by WB, IP, IF and IHC(P)
- Anti-HLA-DO beta Antibody (DOB.L1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for HLA-DOβ Antibody (DOB.L1) for WB and IHC(P) applications. These reagents are now offered in bundles with HLA-DOβ Antibody (DOB.L1) (see ordering information below).
HLA-DOβ Antibody (DOB.L1) is a mouse monoclonal IgG2b kappa light chain antibody that detects the HLA-DO beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). HLA-DOβ (DOB.L1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DO beta protein plays a crucial role in the immune response by regulating the presentation of antigens to CD4+ T cells, which is essential for initiating adaptive immunity. Specifically, HLA-DO beta protein interacts with HLA-DM to facilitate the exchange of peptides in the antigen-binding groove of major histocompatibility complex (MHC) class II molecules. This interaction is vital for ensuring that T cells can recognize and respond to foreign antigens effectively. Proper functioning of HLA-DO beta protein is particularly important in the context of autoimmune diseases, as variations in the genes encoding these molecules have been linked to susceptibility to conditions such as rheumatoid arthritis. Using anti-HLA-DOβ antibody (DOB.L1) in research can provide insights into the mechanisms of antigen presentation and the role of HLA molecules in immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DOβ Antibody (DOB.L1) References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism. | Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DOβ Antibody (DOB.L1) | sc-69739 | 200 µg/ml | $322.00 | |||
HLA-DOβ Antibody (DOB.L1): m-IgG Fc BP-HRP Bundle | sc-525694 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DOβ Antibody (DOB.L1): m-IgG2b BP-HRP Bundle | sc-549623 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DOβ Antibody (DOB.L1) AC | sc-69739 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-DOβ Antibody (DOB.L1) HRP | sc-69739 HRP | 200 µg/ml | $322.00 | |||
HLA-DOβ Antibody (DOB.L1) FITC | sc-69739 FITC | 200 µg/ml | $336.00 | |||
HLA-DOβ Antibody (DOB.L1) PE | sc-69739 PE | 200 µg/ml | $349.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 488 | sc-69739 AF488 | 200 µg/ml | $364.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 546 | sc-69739 AF546 | 200 µg/ml | $364.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 594 | sc-69739 AF594 | 200 µg/ml | $364.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 647 | sc-69739 AF647 | 200 µg/ml | $364.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 680 | sc-69739 AF680 | 200 µg/ml | $364.00 | |||
HLA-DOβ Antibody (DOB.L1) Alexa Fluor® 790 | sc-69739 AF790 | 200 µg/ml | $364.00 |