HLA-DOα Antibody (C-11): sc-515446
- HLA-DOα Antibody (C-11) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 83-127 mapping within an internal region of HLA-DOα of human origin
- Anti-HLA-DO alpha Antibody (C-11) is recommended for detection of HLA-DOα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HLA-DO alpha Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA-DOα Antibody (C-11) for WB applications. These reagents are now offered in bundles with HLA-DOα Antibody (C-11) (see ordering information below).
HLA-DOα Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects HLA-DOα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HLA-DOα antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DOα, a 250 amino acid single-pass membrane protein, plays a crucial role in the immune response by forming a heterodimer with HLA-DOβ. This interaction is vital for modulating the HLA class II restricted antigen presentation pathway, particularly in the context of peptide loading and presentation to CD4+ helper T cells. HLA-DOα′s location within endosomal and lysosomal compartments, as well as presence on the surface of B cells, underscores HLA-DOα′s importance in regulating the dissociation of class II-associated invariant chain peptides (CLIP) and facilitating the binding of exogenous peptides to HLA class II molecules, such as HLA-DR. By maintaining a conformation that favors peptide exchange, HLA-DOα, in conjunction with HLA-DM, ensures effective immune signaling and response, making HLA-DOα a critical component in the adaptive immune system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DOα Antibody (C-11) References:
- Limited polymorphism in the HLA-DOA gene. | Naruse, TK., et al. 1999. Tissue Antigens. 53: 359-65. PMID: 10323340
- Novel polymorphisms in HLA-DOA and HLA-DOB in B-cell malignancies. | van Lith, M., et al. 2002. Immunogenetics. 54: 591-5. PMID: 12439622
- Ectopic expression of HLA-DO in mouse dendritic cells diminishes MHC class II antigen presentation. | Fallas, JL., et al. 2004. J Immunol. 173: 1549-60. PMID: 15265882
- Identification of four novel HLA-DOA alleles, DOA*010106, DOA*0102, DOA*0103, and DOA*0104N, by sequence-based typing*. | Moon, SM., et al. 2005. Tissue Antigens. 66: 242-5. PMID: 16101837
- Detection of aberrant transcription of major histocompatibility complex class II antigen presentation genes in chronic lymphocytic leukaemia identifies HLA-DOA mRNA as a prognostic factor for survival. | Souwer, Y., et al. 2009. Br J Haematol. 145: 334-43. PMID: 19245431
- Exploring the diabetogenicity of the HLA-B18-DR3 CEH: independent association with T1D genetic risk close to HLA-DOA. | Santin, I., et al. 2009. Genes Immun. 10: 596-600. PMID: 19458622
- The HLA-DNA (DZA) gene is correctly expressed as a 1.1 kb mature mRNA transcript. | Young, JA. and Trowsdale, J. 1990. Immunogenetics. 31: 386-8. PMID: 2370084
- Human class II DNA and DOB genes display low sequence variability. | Jonsson, AK. and Rask, L. 1989. Immunogenetics. 29: 411-3. PMID: 2499532
- The human HLA class II alpha chain gene DZ alpha is distinct from genes in the DP, DQ and DR subregions. | Trowsdale, J. and Kelly, A. 1985. EMBO J. 4: 2231-7. PMID: 3000765
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DOα Antibody (C-11) | sc-515446 | 200 µg/ml | $322.00 | |||
HLA-DOα Antibody (C-11): m-IgG Fc BP-HRP Bundle | sc-531371 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DOα Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-525045 | 200 µg Ab, 40 µg BP | $361.00 | |||
HLA-DOα Antibody (C-11): m-IgG1 BP-HRP Bundle | sc-544510 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-DOα Antibody (C-11) AC | sc-515446 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-DOα Antibody (C-11) HRP | sc-515446 HRP | 200 µg/ml | $322.00 | |||
HLA-DOα Antibody (C-11) FITC | sc-515446 FITC | 200 µg/ml | $336.00 | |||
HLA-DOα Antibody (C-11) PE | sc-515446 PE | 200 µg/ml | $349.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 488 | sc-515446 AF488 | 200 µg/ml | $364.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 546 | sc-515446 AF546 | 200 µg/ml | $364.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 594 | sc-515446 AF594 | 200 µg/ml | $364.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 647 | sc-515446 AF647 | 200 µg/ml | $364.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 680 | sc-515446 AF680 | 200 µg/ml | $364.00 | |||
HLA-DOα Antibody (C-11) Alexa Fluor® 790 | sc-515446 AF790 | 200 µg/ml | $364.00 |