HLA-DMβ Antibody (E-8): sc-393548
- HLA-DMβ Antibody (E-8) is a mouse monoclonal IgG1 κ HLA-DMβ antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-263 representing full length HLA-DMβ of human origin
- HLA-DM beta Antibody (E-8) is recommended for detection of HLA-DMβ of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HLA-DM beta Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA-DMβ Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with HLA-DMβ Antibody (E-8) (see ordering information below).
HLA-DMβ Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HLA-DM beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HLA-DMβ (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DMβ protein, also known as DMB or RING7, is a 263 amino acid single-pass type I membrane protein that plays a pivotal role in immune response by facilitating the loading of antigenic peptides onto major histocompatibility complex (MHC) class II molecules. This process is crucial for CD4+ T cell activation, allowing for processed antigen presentation to the immune system. HLA-DMβ exists as a heterodimer with an alpha chain (DMA) and a beta chain (DMB), and HLA-DMβ function is intricately regulated by HLA-DO presence, which modulates the interaction between HLA-DM and MHC class II molecules in B cells. HLA-DMβ gene is located on human chromosome 6p21.32 and consists of approximately 6,442 bases, highlighting HLA-DMβ significance in the genetic landscape of immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DMβ Antibody (E-8) References:
- Identification of a novel HLA-DMB allele (DMB*0107) in the Korean population. | Gu, H., et al. 2005. Tissue Antigens. 65: 393-4. PMID: 15787724
- A new human HLA class II-related locus, DM. | Kelly, AP., et al. 1991. Nature. 353: 571-3. PMID: 1922365
- Genomic organization of HLA-DMA and HLA-DMB. Comparison of the gene organization of all six class II families in the human major histocompatibility complex. | Radley, E., et al. 1994. J Biol Chem. 269: 18834-8. PMID: 8034636
- Limited polymorphism in HLA-DM does not involve the peptide binding groove. | Sanderson, F., et al. 1994. Immunogenetics. 39: 56-8. PMID: 8225438
- Characterization of HLA-DMB polymorphism. | Carrington, M., et al. 1993. Immunogenetics. 38: 446-9. PMID: 8406617
- Evolutionary dynamics of non-coding sequences within the class II region of the human MHC. | Beck, S., et al. 1996. J Mol Biol. 255: 1-13. PMID: 8568858
- Targeting signal and subcellular compartments involved in the intracellular trafficking of HLA-DMB. | Copier, J., et al. 1996. J Immunol. 157: 1017-27. PMID: 8757605
- Three HLA-DMB variants in Korean patients with autoimmune diseases. | Kim, TG., et al. 1996. Hum Immunol. 46: 58-60. PMID: 9157091
- The structure of HLA-DM, the peptide exchange catalyst that loads antigen onto class II MHC molecules during antigen presentation. | Mosyak, L., et al. 1998. Immunity. 9: 377-83. PMID: 9768757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DMβ Antibody (E-8) | sc-393548 | 200 µg/ml | $322.00 | |||
HLA-DMβ Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-530491 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DMβ Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-523990 | 200 µg Ab, 40 µg BP | $361.00 | |||
HLA-DMβ Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544085 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-DMβ Antibody (E-8) AC | sc-393548 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-DMβ Antibody (E-8) HRP | sc-393548 HRP | 200 µg/ml | $322.00 | |||
HLA-DMβ Antibody (E-8) FITC | sc-393548 FITC | 200 µg/ml | $336.00 | |||
HLA-DMβ Antibody (E-8) PE | sc-393548 PE | 200 µg/ml | $349.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 488 | sc-393548 AF488 | 200 µg/ml | $364.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 546 | sc-393548 AF546 | 200 µg/ml | $364.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 594 | sc-393548 AF594 | 200 µg/ml | $364.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 647 | sc-393548 AF647 | 200 µg/ml | $364.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 680 | sc-393548 AF680 | 200 µg/ml | $364.00 | |||
HLA-DMβ Antibody (E-8) Alexa Fluor® 790 | sc-393548 AF790 | 200 µg/ml | $364.00 |