Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HLA-DMβ CRISPR/Cas9 KO Plasmid (h): sc-406436
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HLA-DMβ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HLA-DMβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HLA-DMβ Antibody (E-8): sc-393548
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DMβ CRISPR/Cas9 KO Plasmid (h) | sc-406436 | 20 µg | $397.00 |
Overview
HLA-DMB encodes the beta chain of HLA-DM, a non-classical MHC class II molecule that catalyzes peptide loading onto HLA-DR, -DP, and -DQ by promoting CLIP release and stabilizing high-affinity peptide–MHC II complexes in late endosomal compartments. This editing step is central to antigen processing and presentation pathways that shape CD4+ T cell priming, thymic selection, and peripheral immune tolerance. Altered HLA-DM function can shift the immunopeptidome and influence inflammatory signaling programs linked to autoimmunity and immune dysregulation. As a result, HLA-DMB is widely studied in the context of HLA class II expression networks, interferon-responsive antigen presentation, and disease-associated HLA haplotypes.
HLA-DMβ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HLA-DMB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HLA-DMB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HLA-DMB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HLA-DMβ protein expression.
This CRISPR knockout system enables efficient generation of HLA-DMB-deficient cell models for investigation of HLA-DMβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HLA-DMB exon(s) critical for HLA-DMβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HLA-DMB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HLA-DMβ CRISPR/Cas9 KO Plasmid (h) and HLA-DMβ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HLA-DMB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HLA-DMβ HDR Plasmid (h) and HLA-DMβ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HLA-DMB homology arms to support homology-directed repair at defined HLA-DMB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.