HLA-B/C Antibody (A-3): sc-166668
- HLA-B/C Antibody (A-3) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 126-145 within an internal region of HLA-C of human origin
- HLA-B/C Antibody (A-3) is recommended for detection of HLA-B and HLA-C of human origin, H2-L and H2-Q of mouse origin, and the corresponding rat homologs by WB, IP, IF and ELISA
- Anti-HLA-B/C Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HLA-B/C Antibody (A-3) for WB applications. These reagents are now offered in bundles with HLA-B/C Antibody (A-3) (see ordering information below).
HLA-B/C Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody, specifically designed to recognize and bind human leukocyte antigens HLA-B and HLA-C, also known as MHC class I molecules. These proteins are located on the surface of nearly all nucleated cells, where they play a crucial role in the immune system by presenting endogenous peptide antigens to CD8+ cytotoxic T lymphocytes, enabling immune surveillance and the elimination of infected or malignant cells. The cell-surface localization of HLA-B and HLA-C is essential for effective antigen presentation and immune recognition, facilitating communication between immune cells and maintaining immune homeostasis. Anti-HLA-B/C antibody (A-3) targets a specific epitope between amino acids 126-145 within an internal region of HLA-C, ensuring high specificity and affinity for these native proteins in their natural conformation. Additionally, HLA-B/C monoclonal antibody (A-3) cross-reacts with mouse H2-L and H2-Q, as well as their corresponding rat homologs, making this a versatile tool for multi-species studies. Anti-HLA-B/C antibody (A-3) is effective in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), providing reliable and consistent results for researchers investigating immune responses, autoimmunity, and transplant compatibility. By targeting HLA-B and HLA-C molecules, HLA-B/C monoclonal antibody (A-3) facilitates the study of antigen presentation mechanisms and interactions between different immune cells, advancing our understanding of immune regulation and therapeutic development. Anti-HLA-B/C antibody (A-3) detects a broad range of HLA-B/C alleles, enhancing research utility in both basic and clinical settings, ensuring reproducible outcomes across diverse experimental platforms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-B/C Antibody (A-3) References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism. | Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-B/C Antibody (A-3) | sc-166668 | 200 µg/ml | $322.00 | |||
HLA-B/C Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-527176 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-B/C Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-532549 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-B/C Antibody (A-3) AC | sc-166668 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-B/C Antibody (A-3) HRP | sc-166668 HRP | 200 µg/ml | $322.00 | |||
HLA-B/C Antibody (A-3) FITC | sc-166668 FITC | 200 µg/ml | $336.00 | |||
HLA-B/C Antibody (A-3) PE | sc-166668 PE | 200 µg/ml | $349.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 488 | sc-166668 AF488 | 200 µg/ml | $364.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 546 | sc-166668 AF546 | 200 µg/ml | $364.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 594 | sc-166668 AF594 | 200 µg/ml | $364.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 647 | sc-166668 AF647 | 200 µg/ml | $364.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 680 | sc-166668 AF680 | 200 µg/ml | $364.00 | |||
HLA-B/C Antibody (A-3) Alexa Fluor® 790 | sc-166668 AF790 | 200 µg/ml | $364.00 | |||
HLA-B/C (A-3) Neutralizing Peptide | sc-166668 P | 100 µg/0.5 ml | $69.00 |