HIV-2 Nef Antibody (4B5): sc-57008
- HIV-2 Nef Antibody (4B5) is a rat monoclonal IgG1 HIV-2 Nef antibody provided at 200 µg/ml
- raised against an HIV-2 Nef-TrpE fusion protein
- HIV-2 Nef Antibody (4B5) is recommended for detection of Nef of HIV-2 origin by WB and IP; non cross-reactive with HIV-1
- Anti-HIV-2 Nef Antibody (4B5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIV-2 Nef Antibody (4B5). This work is in progress.
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HIV-2 Nef Antibody (4B5) is a rat monoclonal IgG1 antibody that detects the HIV-2 Nef protein of HIV-2 origin by western blotting (WB) and immunoprecipitation (IP). Anti-HIV-2 Nef antibody (4B5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIV-2 Nef protein plays a crucial role in the viral life cycle, primarily by modulating the host immune response and enhancing viral replication. HIV-2 Nef achieves this by downregulating the expression of major histocompatibility complex (MHC) class I molecules on the surface of infected cells, which helps the virus evade detection by CD8+ cytotoxic T cells. This immune evasion is particularly important as HIV-2 can persist in the host, contributing to the chronic nature of HIV infection and the progression to acquired immunodeficiency syndrome (AIDS). Understanding HIV-2 Nef function is vital for developing therapeutic strategies aimed at targeting HIV-2, especially given that HIV-2 is less transmissible than HIV-1 but poses significant challenges in regions where HIV-2 is endemic, such as West Africa.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIV-2 Nef Antibody (4B5) References:
- High frequency of antibodies against recombinant HIV-2 Nef protein in HIV-2-positive and HIV-negative sera. | Mayer, J., et al. 1992. AIDS. 6: 519-20. PMID: 1616664
- Purification and characterization of human immunodeficiency virus type 1 nef gene product expressed by a recombinant baculovirus. | Matsuura, Y., et al. 1991. Virology. 184: 580-6. PMID: 1909480
- Nef proteins of the human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV) are structurally similar to leucine zipper transcriptional activation factors. | Samuel, KP., et al. 1991. AIDS Res Hum Retroviruses. 7: 697-706. PMID: 1931237
- A possible regulation of negative factor (Nef) activity of human immunodeficiency virus type 1 by the viral protease. | Freund, J., et al. 1994. Eur J Biochem. 223: 589-93. PMID: 8055930
- Purification of an Escherichia coli-expressed Nef protein from the human immunodeficiency virus-type 2. | Du Bois, GC., et al. 1993. AIDS Res Hum Retroviruses. 9: 1225-31. PMID: 8142139
- Subcloning of HIV-2 nef genes in E. coli and immunological reactivity of expressed fusion proteins. | Ulrich, R., et al. 1993. Virus Genes. 7: 229-40. PMID: 8279123
- Human immunodeficiency virus (HIV) Nef is an RNA binding protein in cell-free systems. | Echarri, A., et al. 1996. J Mol Biol. 262: 640-51. PMID: 8876644
- The N-terminal Arg-rich region of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus Nef is involved in RNA binding. | Echarri, A., et al. 1997. Eur J Biochem. 246: 38-44. PMID: 9210463
- Association of human immunodeficiency virus Nef protein with actin is myristoylation dependent and influences its subcellular localization. | Fackler, OT., et al. 1997. Eur J Biochem. 247: 843-51. PMID: 9288906
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-2 Nef Antibody (4B5) | sc-57008 | 200 µg/ml | $316.00 | |||
HIV-2 Nef Antibody (4B5) AC | sc-57008 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HIV-2 Nef Antibody (4B5) HRP | sc-57008 HRP | 200 µg/ml | $316.00 | |||
HIV-2 Nef Antibody (4B5) FITC | sc-57008 FITC | 200 µg/ml | $330.00 | |||
HIV-2 Nef Antibody (4B5) PE | sc-57008 PE | 200 µg/ml | $343.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 488 | sc-57008 AF488 | 200 µg/ml | $357.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 546 | sc-57008 AF546 | 200 µg/ml | $357.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 594 | sc-57008 AF594 | 200 µg/ml | $357.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 647 | sc-57008 AF647 | 200 µg/ml | $357.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 680 | sc-57008 AF680 | 200 µg/ml | $357.00 | |||
HIV-2 Nef Antibody (4B5) Alexa Fluor® 790 | sc-57008 AF790 | 200 µg/ml | $357.00 |