HIV-1 Tat-SF1 Antibody (C-4): sc-514351
- HIV-1 Tat-SF1 Antibody (C-4) is a mouse monoclonal IgG2b κ HIV-1 Tat-SF1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-23 at the N-terminus of HIV-1 Tat-SF1 of mouse origin
- HIV-1 Tat-SF1 Antibody (C-4) is recommended for detection of HIV-1 Tat-SF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HIV-1 Tat-SF1 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HIV-1 Tat-SF1 Antibody (C-4) for WB applications. These reagents are now offered in bundles with HIV-1 Tat-SF1 Antibody (C-4) (see ordering information below).
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HIV-1 Tat-SF1 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects HIV-1 Tat-SF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIV-1 Tat-SF1 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIV-1 Tat-SF1, also known as HIV-1 Tat-specific factor 1, is a crucial phosphoprotein involved in transcriptional elongation, which is essential for efficient expression of HIV-1 genes. HIV-1 Tat-SF1 is predominantly localized in the nucleus, where HIV-1 Tat-SF1 plays a vital role in coupling transcription and splicing by forming complexes with U small nuclear ribonucleoproteins (U snRNPs). HIV-1 Tat-SF1′s interaction with other proteins, such as Tat, P-TEFb, and Spt5, enhances transcriptional activity of the HIV-1 virus, making HIV-1 Tat-SF1 a key player in the viral life cycle. HIV-1 Tat-SF1 contains two RNA recognition motif (RRM) domains and an acidic C-terminal motif, which are critical for function and interaction with RNA and other proteins. Expression of HIV-1 Tat-SF1 is upregulated by HIV-1 proteins Nef and gp120, further highlighting HIV-1 Tat-SF1′s importance in HIV-1 pathogenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIV-1 Tat-SF1 Antibody (C-4) References:
- A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription. | Parada, CA. and Roeder, RG. 1999. EMBO J. 18: 3688-701. PMID: 10393184
- Tat-SF1 protein associates with RAP30 and human SPT5 proteins. | Kim, JB., et al. 1999. Mol Cell Biol. 19: 5960-8. PMID: 10454543
- Relief of two built-In autoinhibitory mechanisms in P-TEFb is required for assembly of a multicomponent transcription elongation complex at the human immunodeficiency virus type 1 promoter. | Fong, YW. and Zhou, Q. 2000. Mol Cell Biol. 20: 5897-907. PMID: 10913173
- Nef triggers a transcriptional program in T cells imitating single-signal T cell activation and inducing HIV virulence mediators. | Simmons, A., et al. 2001. Immunity. 14: 763-77. PMID: 11420046
- Stimulatory effect of splicing factors on transcriptional elongation. | Fong, YW. and Zhou, Q. Nature. 414: 929-33. PMID: 11780068
- Comparative and evolutionary analysis of the rhesus macaque extended MHC class II region. | Sudbrak, R., et al. 2003. Immunogenetics. 54: 699-704. PMID: 12557056
- p54(nrb) associates with the 5' splice site within large transcription/splicing complexes. | Kameoka, S., et al. 2004. EMBO J. 23: 1782-91. PMID: 15057275
- Soluble HIV-1 gp120 enhances HIV-1 replication in non-dividing CD4+ T cells, mediated via cell signaling and Tat cofactor overexpression. | Missé, D., et al. 2005. AIDS. 19: 897-905. PMID: 15905670
- The HIV-1 Tat cellular coactivator Tat-SF1 is a general transcription elongation factor. | Li, XY. and Green, MR. 1998. Genes Dev. 12: 2992-6. PMID: 9765201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-1 Tat-SF1 Antibody (C-4) | sc-514351 | 200 µg/ml | $322.00 | |||
HIV-1 Tat-SF1 Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-524692 | 200 µg Ab, 40 µg BP | $361.00 | |||
HIV-1 Tat-SF1 Antibody (C-4): m-IgG2b BP-HRP Bundle | sc-549174 | 200 µg Ab; 10 µg BP | $361.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) AC | sc-514351 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) HRP | sc-514351 HRP | 200 µg/ml | $322.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) FITC | sc-514351 FITC | 200 µg/ml | $336.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) PE | sc-514351 PE | 200 µg/ml | $349.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 488 | sc-514351 AF488 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 546 | sc-514351 AF546 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 594 | sc-514351 AF594 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 647 | sc-514351 AF647 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 680 | sc-514351 AF680 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 Antibody (C-4) Alexa Fluor® 790 | sc-514351 AF790 | 200 µg/ml | $364.00 | |||
HIV-1 Tat-SF1 (C-4) Neutralizing Peptide | sc-514351 P | 100 µg/0.5 ml | $69.00 |