Date published: 2025-10-15

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HIV-1 Nef Antibody (2F2): sc-130519

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Datasheets
  • HIV-1 Nef Antibody (2F2) is a mouse monoclonal IgG1 provided at 100 µg/ml
  • raised against recombinant Nef protein of HIV-1 (BRU) origin with epitope mapping to amino acids 150-158
  • recommended for detection of Nef of HIV-1 origin by WB, IF and IHC(P)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIV-1 Nef Antibody (2F2). This work is in progress.

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    HIV-1 Nef Antibody (2F2) is a mouse monoclonal IgG1 antibody that detects HIV-1 Nef in HIV-1 by applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. HIV-1 Nef is a crucial peripheral membrane protein that plays a significant role in the pathogenesis of HIV by modulating host cell signaling pathways and immune responses. Anti-HIV-1 Nef antibody (2F2) recognizes a protein that interacts with various cellular proteins, particularly Src-family tyrosine kinases and GTPase activators, through its SH3-binding domain. This interaction is vital for HIV-1 Nef′s function in manipulating the host′s immune system, as HIV-1 Nef facilitates the downregulation of CD4, a critical receptor on helper T cells, by promoting its rapid endocytosis. HIV-1 Nef′s structure includes two relatively unstructured loops that are essential for engagement with proteins involved in membrane trafficking, allowing control over the distribution of transmembrane proteins and evasion of immune detection. By inhibiting MHC class II-restricted peptide presentation, HIV-1 Nef effectively reduces the surface expression of mature MHC class II molecules while increasing levels of immature MHC class II associated with invariant chains. This unique capability makes HIV-1 Nef the only HIV-1 gene product that can perform such immune evasion, underscoring its importance in the viral life cycle and the challenges for therapeutic interventions.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    HIV-1 Nef Antibody (2F2) References:

    1. HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck.  |  Pene-Dumitrescu, T., et al. 2012. BMC Chem Biol. 12: 1. PMID: 22420777
    2. How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4.  |  Ren, X., et al. 2014. Elife. 3: e01754. PMID: 24473078
    3. HIV-1 Nef and Vpu are functionally redundant broad-spectrum modulators of cell surface receptors, including tetraspanins.  |  Haller, C., et al. 2014. J Virol. 88: 14241-57. PMID: 25275127
    4. Differential Ability of Primary HIV-1 Nef Isolates To Downregulate HIV-1 Entry Receptors.  |  Toyoda, M., et al. 2015. J Virol. 89: 9639-52. PMID: 26178998
    5. D186/D190 is an allele-dependent determinant of HIV-1 Nef function.  |  Imle, A., et al. 2016. Virology. 498: 44-56. PMID: 27560372
    6. HIV-1 Nef promotes migration and chemokine synthesis of human basophils and mast cells through the interaction with CXCR4.  |  Rossi, FW., et al. 2016. Clin Mol Allergy. 14: 15. PMID: 27822141
    7. A Highly Conserved Residue in HIV-1 Nef Alpha Helix 2 Modulates Protein Expression.  |  Johnson, AL., et al. 2016. mSphere. 1: PMID: 27840851
    8. HIV-1 Nef Hijacks Lck and Rac1 Endosomal Traffic To Dually Modulate Signaling-Mediated and Actin Cytoskeleton-Mediated T Cell Functions.  |  Del Río-Iñiguez, I., et al. 2018. J Immunol. 201: 2624-2640. PMID: 30282749
    9. HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300.  |  Zhou, F., et al. 2018. J Neuroinflammation. 15: 303. PMID: 30382871
    10. Modelling and in vitro testing of the HIV-1 Nef fitness landscape.  |  Barton, JP., et al. 2019. Virus Evol. 5: vez029. PMID: 31392033

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    HIV-1 Nef Antibody (2F2)

    sc-130519
    100 µg/ml
    $316.00