HIV-1 Nef Antibody (01-009): sc-65909
- HIV-1 Nef Antibody (01-009) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against amino acids 195-205 of Nef of HIV-1 origin
- recommended for detection of Nef of HIV-1 origin by WB, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIV-1 Nef Antibody (01-009). This work is in progress.
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HIV-1 Nef Antibody (01-009) is a mouse monoclonal IgG1 antibody that detects HIV-1 Nef in HIV-1 through applications like western blotting (WB), immunofluorescence (IF), and immunohistochemistry. HIV-1 Nef is a crucial peripheral membrane protein that plays a significant role in HIV pathogenesis by modulating host cell signaling pathways and immune responses. Anti-HIV-1 Nef antibody (01-009) recognizes a protein that interacts with various cellular proteins, particularly Src-family tyrosine kinases and GTPase activators, through its SH3-binding domain. This interaction is vital for Nef′s function in manipulating the host cell environment, as HIV-1 Nef affects the phosphoinositide 3-kinase sphingomyelinase signaling pathways, leading to CD4 downregulation on infected T cell surfaces. By promoting rapid CD4 endocytosis, HIV-1 Nef aids in immune response evasion and enhances viral replication. HIV-1 Nef′s structural features, including two relatively unstructured loops, facilitate binding to cellular proteins involved in membrane trafficking, which is essential for controlling transmembrane protein distribution and ultimately contributes to viral immune detection escape. These unique properties of HIV-1 Nef make this protein a critical target for research aimed at understanding HIV pathogenesis and developing therapeutic strategies.
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HIV-1 Nef Antibody (01-009) References:
- Immunoregulatory effects of HIV-1 Nef protein. | Quaranta, MG., et al. 2009. Biofactors. 35: 169-74. PMID: 19449444
- HIV-1 Nef blocks autophagy in human astrocytes. | Sardo, L., et al. 2015. Cell Cycle. 14: 3781-2. PMID: 26697833
- The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4+ T cell apoptosis. | Jacob, RA., et al. 2017. Virology. 509: 1-10. PMID: 28577469
- A Novel HIV-1 Nef Mutation in a Primary Pediatric Isolate Impairs MHC-Class I Downregulation and Cytopathicity. | Ali, A., et al. 2020. AIDS Res Hum Retroviruses. 36: 122-130. PMID: 31571497
- Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity. | Jin, SW., et al. 2019. Cell Rep. 29: 1449-1457.e5. PMID: 31693887
- An HIV-1 Nef genotype that diminishes immune control mediated by protective human leucocyte antigen alleles. | Mwimanzi, F., et al. 2020. AIDS. 34: 1325-1330. PMID: 32590431
- HIV-1 Nef Interacts with LMP7 To Attenuate Immunoproteasome Formation and Major Histocompatibility Complex Class I Antigen Presentation. | Yang, Y., et al. 2020. mBio. 11: PMID: 33109760
- HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity. | Chai, Q., et al. 2021. Cell Rep. 36: 109514. PMID: 34380030
- HIV-1 Nef Protein Affects Cytokine and Extracellular Vesicles Production in the GEN2.2 Plasmacytoid Dendritic Cell Line. | Aiello, A., et al. 2021. Viruses. 14: PMID: 35062278
- HIV-1 Nef and host cell protein kinases. | Saksela, K. 1997. Front Biosci. 2: d606-18. PMID: 9388166
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-1 Nef Antibody (01-009) | sc-65909 | 100 µg/ml | $316.00 |