HIV-1 Nef Antibody (01-004): sc-65907

HIV-1 Nef Antibody (01-004) is a mouse monoclonal IgG1 antibody that detects HIV-1 Nef in HIV-1 through applications including western blotting (WB), immunofluorescence (IF), and immunohistochemistry. HIV-1 Nef is a crucial peripheral membrane protein that plays a significant role in HIV pathogenesis by modulating host cell signaling pathways and immune responses. Anti-HIV-1 Nef antibody (01-004) recognizes a protein that interacts with various cellular proteins, particularly Src-family tyrosine kinases and GTPase activators, through its SH3-binding domain. This interaction is vital for Nef′s function in manipulating the host cell environment, as HIV-1 Nef affects the phosphoinositide 3-kinase sphingomyelinase signaling pathways and facilitates CD4 downregulation, a critical receptor on T cells. By promoting rapid CD4 endocytosis from the cell surface, HIV-1 Nef effectively diminishes the immune system′s ability to recognize and respond to the virus, thereby enhancing viral persistence and evasion of immune surveillance. Additionally, Nef′s structural features, including two relatively unstructured loops, enable engagement with proteins involved in membrane trafficking, further supporting a role in altering transmembrane protein distribution. HIV-1 Nef′s unique ability to inhibit MHC class II-restricted peptide presentation underscores importance as the only HIV-1 gene product capable of modulating this immune pathway, ultimately contributing to viral pathogenicity and acquired immune deficiency syndrome (AIDS) progression.

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HIV-1 Nef Antibody (01-004) References:

1. HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System.  |  Shi, J., et al. 2018. J Virol. 92: PMID: 29514909
2. HIV-1 Nef promotes cell proliferation and microRNA dysregulation in lung cells.  |  Santerre, M., et al. 2019. Cell Cycle. 18: 130-142. PMID: 30563405
3. HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation.  |  Kumari, S., et al. 2019. Life Sci. 229: 13-20. PMID: 30953643
4. HIV-1 Impairment via UBE3A and HIV-1 Nef Interactions Utilizing the Ubiquitin Proteasome System.  |  Pyeon, D., et al. 2019. Viruses. 11: PMID: 31783587
5. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner.  |  Castro-Gonzalez, S., et al. 2021. Autophagy. 17: 553-577. PMID: 32097085
6. HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane.  |  Li, WF., et al. 2020. J Biol Chem. 295: 5163-5174. PMID: 32144207
7. HIV-1 Nef promotes ubiquitination and proteasomal degradation of p53 tumor suppressor protein by using E6AP.  |  Ali, A., et al. 2020. Biochem Biophys Res Commun. 529: 1038-1044. PMID: 32819562
8. Direct interaction between ABCA1 and HIV-1 Nef: Molecular modeling and virtual screening for inhibitors.  |  Adzhubei, AA., et al. 2021. Comput Struct Biotechnol J. 19: 3876-3884. PMID: 34584633
9. Neutron Reflectometry and Molecular Simulations Demonstrate HIV-1 Nef Homodimer Formation on Model Lipid Bilayers.  |  Heinrich, F., et al. 2023. J Mol Biol. 435: 168009. PMID: 36773691
10. PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication.  |  Emert-Sedlak, LA., et al. 2024. Cell Chem Biol. 31: 658-668.e14. PMID: 38508197

The preferred HIV-1 p24 antibody is HIV-1 p24 Antibody (24-4): sc-69728.