HIV-1 Nef Antibody (01-002): sc-65905
- HIV-1 Nef Antibody (01-002) is a mouse monoclonal IgG1, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 35-50 of Nef of HIV-1 origin
- recommended for detection of Nef of HIV-1 origin by WB, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIV-1 Nef Antibody (01-002). This work is in progress.
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HIV-1 Nef Antibody (01-002) is a mouse monoclonal IgG1 antibody that detects HIV-1 Nef in HIV-1 through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. HIV-1 Nef is a crucial peripheral membrane protein that plays a significant role in the pathogenesis of HIV by modulating host cell signaling pathways and immune responses. One of the key aspects of HIV-1 Nef is its ability to interact with various cellular proteins, particularly Src-family tyrosine kinases and GTPase activators, through its SH3-binding domain. This interaction is vital for Nef′s function in manipulating the host cell environment, as HIV-1 Nef affects the phosphoinositide 3-kinase sphingomyelinase signaling pathways, leading to the downregulation of CD4 on the surface of infected T cells. By promoting the rapid endocytosis of CD4, HIV-1 Nef not only aids in the evasion of the host immune response but also enhances viral replication. Furthermore, HIV-1 Nef′s structural features, including its two relatively unstructured loops, facilitate binding to cellular proteins involved in membrane trafficking, which is essential for controlling the distribution of transmembrane proteins and ultimately for the virus′s ability to persist in the host. The unique properties of HIV-1 Nef make this protein a critical target for research aimed at understanding HIV pathogenesis and developing therapeutic strategies.
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HIV-1 Nef Antibody (01-002) References:
- HIV-1 Nef in macrophage-mediated disease pathogenesis. | Lamers, SL., et al. 2012. Int Rev Immunol. 31: 432-50. PMID: 23215766
- HIV-1 Nef impairs key functional activities in human macrophages through CD36 downregulation. | Olivetta, E., et al. 2014. PLoS One. 9: e93699. PMID: 24705461
- HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation. | Rosa, A., et al. 2015. Nature. 526: 212-7. PMID: 26416734
- HIV-1 Nef hijacks clathrin coats by stabilizing AP-1:Arf1 polygons. | Shen, QT., et al. 2015. Science. 350: aac5137. PMID: 26494761
- HIV-1 Nef Signaling in Intestinal Mucosa Epithelium Suggests the Existence of an Active Inter-kingdom Crosstalk Mediated by Exosomes. | Felli, C., et al. 2017. Front Microbiol. 8: 1022. PMID: 28642743
- Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding. | Moroco, JA., et al. 2018. J Mol Biol. 430: 310-321. PMID: 29258818
- HIV-1 Nef-POTEE; A novel interaction modulates macrophage dissemination via mTORC2 signaling pathway. | Vekariya, U., et al. 2018. Life Sci. 214: 158-166. PMID: 30391463
- HIV-1 Nef Targets HDAC6 to Assure Viral Production and Virus Infection. | Marrero-Hernández, S., et al. 2019. Front Microbiol. 10: 2437. PMID: 31736889
- HIV-1 Nef hijacks both exocytic and endocytic pathways of host intracellular trafficking through differential regulation of Rab GTPases. | Kumari, S., et al. 2022. Biol Cell. 114: 276-292. PMID: 35713972
- Extracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells. | Dubrovsky, L., et al. 2022. Cell Rep. 41: 111674. PMID: 36417867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-1 Nef Antibody (01-002) | sc-65905 | 100 µg/ml | $316.00 |