HIV-1 Nef Antibody (01-001): sc-65904
- HIV-1 Nef Antibody (01-001) is a mouse monoclonal IgG1 HIV-1 Nef antibody, cited in 2 publications, provided at 100 µg/ml
- raised against amino acids 31-35 of Nef of HIV-1 origin
- recommended for detection of Nef of HIV-1 origin by WB, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIV-1 Nef Antibody (01-001). This work is in progress.
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HIV-1 Nef Antibody (01-001) is a mouse monoclonal IgG1 antibody that detects HIV-1 Nef by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-HIV-1 Nef antibody (01-001) is available as a non-conjugated format. HIV-1 Nef, also known as the Negative Regulatory Factor, plays a crucial role in HIV pathogenesis by modulating host immune response. HIV-1 Nef functions as a peripheral membrane protein that interacts with various cellular proteins, influencing signaling pathways vital for viral survival and replication. HIV-1 Nef downregulates CD4, a key receptor on T cells, by promoting endocytosis, thereby evading immune detection. This action allows viral persistence by reducing immune response effectiveness. HIV-1 Nef interacts with Src-family tyrosine kinases and GTPase activators, essential for altering transmembrane protein distribution and facilitating immune evasion. Myristoylated HIV-1 Nef localizes in the cytoplasm, Golgi apparatus, and plasma membrane, while non-myristoylated HIV-1 Nef remains restricted to the cytoplasm. This differential localization enables interactions with host cell machinery and manipulation of cellular processes favoring viral replication. Understanding HIV-1 Nef function and interactions remains vital for developing therapeutic strategies against HIV, as HIV-1 Nef represents a key target for disrupting viral immune evasion.
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HIV-1 Nef Antibody (01-001) References:
- HIV-1 Nef: a master manipulator of the membrane trafficking machinery mediating immune evasion. | Pawlak, EN. and Dikeakos, JD. 2015. Biochim Biophys Acta. 1850: 733-41. PMID: 25585010
- HIV-1 Nef-induced cardiotoxicity through dysregulation of autophagy. | Gupta, MK., et al. 2017. Sci Rep. 7: 8572. PMID: 28819214
- Structure, function, and inhibitor targeting of HIV-1 Nef-effector kinase complexes. | Staudt, RP., et al. 2020. J Biol Chem. 295: 15158-15171. PMID: 32862141
- Research Progress of HIV-1 Nef Inhibitors. | Lv, Y., et al. 2020. AIDS Rev. 22: 221-226. PMID: 33105470
- HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells. | Fittje, P., et al. 2022. PLoS Pathog. 18: e1010572. PMID: 35749424
- Antiretroviral Drug Discovery Targeting the HIV-1 Nef Virulence Factor. | Emert-Sedlak, LA., et al. 2022. Viruses. 14: PMID: 36146831
- Novel role of HIV-1 Nef in regulating the ubiquitination of cellular proteins. | Ghaly, M., et al. 2023. Front Cell Infect Microbiol. 13: 1106591. PMID: 36968110
- HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors. | da Silva-Januário, ME., et al. 2023. Mol Cell Proteomics. 22: 100676. PMID: 37940003
- PROTAC induces HIV-1 Nef degradation. | Crunkhorn, S. 2024. Nat Rev Drug Discov. 23: 340. PMID: 38575781
- HIV-1 Nef is carried on the surface of extracellular vesicles. | Vanpouille, C., et al. 2024. J Extracell Vesicles. 13: e12478. PMID: 39016173
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-1 Nef Antibody (01-001) | sc-65904 | 100 µg/ml | $316.00 |