Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone H1X CRISPR/Cas9 KO Plasmid (h): sc-411232
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H1X CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Histone H1X genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H1X Antibody (E-5): sc-514856
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H1X CRISPR/Cas9 KO Plasmid (h) | sc-411232 | 20 µg | $397.00 |
Overview
H1FX encodes histone H1X, a replication-independent linker histone variant that binds nucleosomal DNA and contributes to higher-order chromatin compaction and genome organization. By modulating chromatin accessibility, H1X influences transcriptional programs, DNA replication timing, and the coordination of DNA damage sensing and repair processes. Altered linker histone composition can shift epigenetic states and impact pathways governing cell-cycle control, differentiation, and stress responses. Dysregulation of chromatin architecture and histone variant balance is frequently observed in cancer and other diseases with epigenomic instability, making H1FX a useful node for mechanistic studies of nuclear structure–function relationships.
Histone H1X CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the H1FX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the H1FX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the H1FX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Histone H1X protein expression.
This CRISPR knockout system enables efficient generation of H1FX-deficient cell models for investigation of Histone H1X signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting H1FX exon(s) critical for Histone H1X function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple H1FX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Histone H1X CRISPR/Cas9 KO Plasmid (h) and Histone H1X CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the H1FX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Histone H1X HDR Plasmid (h) and Histone H1X HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by H1FX homology arms to support homology-directed repair at defined H1FX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.