Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone H10 CRISPR/Cas9 KO Plasmid (m): sc-420754
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Histone H10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H10 Antibody (34): sc-56695
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H10 CRISPR/Cas9 KO Plasmid (m) | sc-420754 | 20 µg | $397.00 |
Overview
Mouse H1f0 encodes histone H1⁰, a differentiation-associated linker histone that binds nucleosomal DNA to stabilize higher-order chromatin compaction and influence genome accessibility. By modulating nucleosome spacing and chromatin dynamics, H1⁰ contributes to epigenetic control of transcriptional programs during cell cycle exit, lineage commitment, and cellular senescence. Altered H1⁰ abundance or distribution has been linked to changes in chromatin state, genome stability, and aberrant transcriptional regulation observed across cancer biology and other disorders involving disrupted differentiation. As a chromatin architectural protein, H1⁰ is frequently studied in pathways governing heterochromatin organization, DNA damage responses, and long-range gene regulation.
Histone H10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the H1f0 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the H1f0 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the H1f0 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Histone H10 protein expression.
This CRISPR knockout system enables efficient generation of H1f0-deficient cell models for investigation of Histone H10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting H1f0 exon(s) critical for Histone H10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple H1f0 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Histone H10 CRISPR/Cas9 KO Plasmid (m) and Histone H10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the H1f0 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Histone H10 HDR Plasmid (m) and Histone H10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by H1f0 homology arms to support homology-directed repair at defined H1f0 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.