Histone H1⁰ Antibody (34): sc-56695

Histone H10 Antibody (34) is a mouse monoclonal IgG1 kappa light chain antibody that detects Histone H10 in mouse, rat, human, bovine, and Xenopus samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Histone H10 antibody (34) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Histone H10, a member of the H1 family of linker histones, plays a crucial role in chromatin structure and function by interacting with linker DNA between nucleosomes, facilitating DNA compaction into higher-order chromatin. Histone H10 is expressed in cells undergoing terminal differentiation or those with low rates of division, distinguishing this protein from other somatic H1 histones typically found in proliferating cells. Histone H10 is derived from the intronless gene H1F0, located on chromosome 22q13.1 in humans, and is widely expressed across various tissues in most vertebrates, highlighting an essential role in cellular processes. Post-translational modifications of Histone H10, such as methylation, acetylation, and phosphorylation, can significantly influence gene expression and chromatin dynamics, making Histone H10 (34) monoclonal antibody vital for understanding epigenetic regulation and cellular differentiation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Histone H1⁰ Antibody (34) References:

1. Histone H1 diversity: bridging regulatory signals to linker histone function.  |  Khochbin, S. 2001. Gene. 271: 1-12. PMID: 11410360
2. H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors.  |  Gabrilovich, DI., et al. 2002. J Leukoc Biol. 72: 285-96. PMID: 12149419
3. Association of histone H1(0) with a gene repressed during liver development.  |  Roche, J., et al. Nature. 314: 197-8. PMID: 2579343
4. Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene.  |  Doenecke, D. and Tönjes, R. 1986. J Mol Biol. 187: 461-4. PMID: 3084796
5. Induction of histone H1(0) differs with different treatments among different cell lines.  |  Hall, JM., et al. 1985. FEBS Lett. 189: 92-6. PMID: 4040875
6. Accumulation of histone H1(0) during chemically induced differentiation of murine neuroblastoma cells.  |  Pieler, C., et al. 1981. Eur J Biochem. 115: 329-33. PMID: 6263627
7. Variation of H1(0) content throughout the cell cycle in regenerating rat liver.  |  Gorka, C., et al. 1995. Exp Cell Res. 217: 528-33. PMID: 7698253
8. All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6.  |  Albig, W., et al. 1993. Genomics. 16: 649-54. PMID: 8325638
9. Histone Sequence Database: a compilation of highly-conserved nucleoprotein sequences.  |  Baxevanis, AD. and Landsman, D. 1996. Nucleic Acids Res. 24: 245-7. PMID: 8594591
10. Differential recognition of histone H10 by monoclonal antibodies during cell differentiation and the arrest of cell proliferation.  |  Gorka, C., et al. 1998. J Biol Chem. 273: 1208-15. PMID: 9422788
11. The microheterogeneity of the mammalian H1(0) histone. Evidence for an age-dependent deamidation.  |  Lindner, H., et al. 1998. J Biol Chem. 273: 13324-30. PMID: 9582379
12. The histone H1(0) contains multiple sequence elements for nuclear targeting.  |  Schwamborn, K., et al. 1998. Exp Cell Res. 244: 206-17. PMID: 9770363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Histone H1⁰ Antibody (34) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Histone H1⁰ Antibody (34) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Histone H1⁰ Antibody (34) Specifications

Alternate Product Name: Histone H10

Product Clone Name: 34

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 20-30

Epitope Target: Histone H1⁰

Epitope Species: bovine

Product Reactivity/Detection: Histone H1⁰

Species Reactivity/Detection: mouse, rat, human, bovine, Xenopus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Histone H1⁰ Antibody (34): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin