HIRIP3 Antibody (D-10): sc-376814
- HIRIP3 Antibody (D-10) is a mouse monoclonal IgG2a κ HIRIP3 antibody provided at 200 µg/ml
- raised against amino acids 1-100 mapping at the N-terminus of HIRIP3 of human origin
- HIRIP3 Antibody (D-10) is recommended for detection of HIRIP3 of human origin by WB, IP, IF and ELISA
- Anti-HIRIP3 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HIRIP3 Antibody (D-10) for WB applications. These reagents are now offered in bundles with HIRIP3 Antibody (D-10) (see ordering information below).
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HIRIP3 Antibody (D-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects HIRIP3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIRIP3 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIRIP3, also known as HIRA interacting protein 3, is a 556 amino acid nuclear protein that plays a crucial role in chromatin function and histone metabolism. HIRIP3 is heavily phosphorylated, particularly by casein kinase II, and is present throughout the cell cycle, indicating importance in cellular processes. HIRIP3 interacts directly with HIRA histone chaperone and shows weaker interactions with core histones, specifically Histone H2B and Histone H3. The C-terminal region of HIRIP3, approximately 60 amino acids long, is highly conserved across vertebrates and contains residues that are invariantly charged, polar, and hydrophobic, which may be essential for functional interactions. Additionally, two isoforms of HIRIP3 arise from alternative splicing, with isoform 1 predominantly expressed in skeletal muscle and isoform 2 found in the liver and heart. Human homologs of HIRA are implicated in developmental disorders such as DiGeorge syndrome, highlighting HIRIP3′s significance in both normal physiology and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIRIP3 Antibody (D-10) References:
- Identification of human and mouse HIRA-interacting protein-5 (HIRIP5), two mammalian representatives in a family of phylogenetically conserved proteins with a role in the biogenesis of Fe/S proteins. | Lorain, S., et al. 2001. Biochim Biophys Acta. 1517: 376-83. PMID: 11342215
- Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth. | Ahmad, A., et al. 2005. J Biol Chem. 280: 32090-100. PMID: 16024922
- Evolutionary turnover of mammalian transcription start sites. | Frith, MC., et al. 2006. Genome Res. 16: 713-22. PMID: 16687732
- Chz1, a nuclear chaperone for histone H2AZ. | Luk, E., et al. 2007. Mol Cell. 25: 357-68. PMID: 17289584
- HIRIP3 is a nuclear phosphoprotein interacting with and phosphorylated by the serine-threonine kinase CK2. | Assrir, N., et al. 2007. Biol Chem. 388: 391-8. PMID: 17391060
- Core histones and HIRIP3, a novel histone-binding protein, directly interact with WD repeat protein HIRA. | Lorain, S., et al. 1998. Mol Cell Biol. 18: 5546-56. PMID: 9710638
- HIRA, a mammalian homologue of Saccharomyces cerevisiae transcriptional co-repressors, interacts with Pax3. | Magnaghi, P., et al. 1998. Nat Genet. 20: 74-7. PMID: 9731536
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIRIP3 Antibody (D-10) | sc-376814 | 200 µg/ml | $316.00 | |||
HIRIP3 Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-529938 | 200 µg Ab; 10 µg BP | $354.00 | |||
HIRIP3 Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-523103 | 200 µg Ab, 40 µg BP | $354.00 | |||
HIRIP3 Antibody (D-10): m-IgG2a BP-HRP Bundle | sc-547404 | 200 µg Ab; 10 µg BP | $354.00 | |||
HIRIP3 Antibody (D-10) AC | sc-376814 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HIRIP3 Antibody (D-10) HRP | sc-376814 HRP | 200 µg/ml | $316.00 | |||
HIRIP3 Antibody (D-10) FITC | sc-376814 FITC | 200 µg/ml | $330.00 | |||
HIRIP3 Antibody (D-10) PE | sc-376814 PE | 200 µg/ml | $343.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 488 | sc-376814 AF488 | 200 µg/ml | $357.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 546 | sc-376814 AF546 | 200 µg/ml | $357.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 594 | sc-376814 AF594 | 200 µg/ml | $357.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 647 | sc-376814 AF647 | 200 µg/ml | $357.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 680 | sc-376814 AF680 | 200 µg/ml | $357.00 | |||
HIRIP3 Antibody (D-10) Alexa Fluor® 790 | sc-376814 AF790 | 200 µg/ml | $357.00 |