Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hippi CRISPR/Cas9 KO Plasmid (h): sc-412438
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hippi CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hippi genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hippi Antibody (D-6): sc-390120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hippi CRISPR/Cas9 KO Plasmid (h) | sc-412438 | 20 µg | $397.00 |
Overview
IFT57 encodes a core component of the intraflagellar transport complex B (IFT-B), supporting anterograde trafficking of cargo required for primary cilium assembly and maintenance. Through its role in ciliogenesis, IFT57 contributes to cilium-dependent signal transduction pathways, including Hedgehog and other receptor-mediated processes that coordinate development, cell cycle progression, and tissue homeostasis. Disruption of IFT-B function is linked to ciliopathy-associated phenotypes and has been implicated in disorders affecting skeletal, renal, and retinal biology, making IFT57 a useful target for mechanistic studies of ciliary transport. In human cells, loss of IFT57 is commonly used to interrogate how impaired ciliary architecture rewires signaling outputs and organelle-dependent trafficking.
Hippi CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IFT57 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IFT57 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IFT57 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hippi protein expression.
This CRISPR knockout system enables efficient generation of IFT57-deficient cell models for investigation of Hippi signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IFT57 exon(s) critical for Hippi function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IFT57 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hippi CRISPR/Cas9 KO Plasmid (h) and Hippi CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IFT57 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hippi HDR Plasmid (h) and Hippi HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IFT57 homology arms to support homology-directed repair at defined IFT57 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.