HIP-55 Antibody (F-9): sc-398351

HIP-55 Antibody (F-9) is a mouse monoclonal IgG2a antibody that detects HIP-55 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIP-55, also known as HPK1-interacting protein of 55 kDa, ABP1, SH3P7, or DBNL, is a 430 amino acid cytoplasmic protein that plays a crucial role in the immune response by acting as an actin-binding adapter protein. HIP-55 is essential for the formation and function of the immunological synapse, where HIP-55 regulates T-cell activation by linking T-cell receptors to the actin cytoskeleton, thereby facilitating gene activation and endocytic processes. The presence of both an ADF-H domain and a SH3 domain in HIP-55 allows interaction with other proteins, such as SHANK2, SHANK3, and PRAM-1, which are vital for maintaining cellular signaling pathways. Additionally, HIP-55 is subject to degradation by caspases during apoptosis, highlighting HIP-55′s importance in cellular processes and the regulation of immune responses. With an ability to bind F-actin without participating in actin polymerization, capping, or bundling, HIP-55 serves as a key player in the dynamic regulation of the cytoskeleton, making anti-HIP-55 antibody (F-9) an invaluable tool for researchers studying T-cell biology and actin dynamics.

The preferred HIP-55 antibody is HIP-55 Antibody (E-11): sc-398498.