HIP-55 Antibody (E-11): sc-398498
- HIP-55 Antibody (E-11) is a mouse monoclonal IgG2a κ HIP-55 antibody provided at 200 µg/ml
- raised against amino acids 100-186 mapping near the N-terminus of HIP-55 of human origin
- HIP-55 Antibody (E-11) is recommended for detection of HIP-55 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HIP-55 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HIP-55 (E-11): sc-398498.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HIP-55 Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with HIP-55 Antibody (E-11) (see ordering information below).
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HIP-55 Antibody (E-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects HIP-55 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HIP-55 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIP-55, also known as HPK1-interacting protein of 55 kDa, ABP1, SH3P7, or DBNL (drebrin-like), is a 430 amino acid cytoplasmic protein that plays a crucial role in the regulation of T-cell activation. HIP-55 acts as an actin-binding adapter protein, linking T-cell receptors to the actin cytoskeleton, which is essential for the formation of the immunological synapse. This interaction is vital for the transduction of signals that lead to gene activation and endocytic processes, ultimately influencing T-cell responses during immune reactions. Additionally, HIP-55 is involved in the binding of F-actin, although HIP-55 does not participate in actin polymerization, capping, or bundling. The presence of both an ADF-H domain and a SH3 domain allows HIP-55 to interact with other proteins such as SHANK2, SHANK3, and PRAM-1, further emphasizing its role in cellular signaling pathways. Notably, HIP-55 is subject to degradation by caspases during apoptosis, highlighting its importance in both cell survival and immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIP-55 Antibody (E-11) References:
- Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin. | Kessels, MM., et al. 2001. J Cell Biol. 153: 351-66. PMID: 11309416
- Caspase-mediated cleavage of actin-binding and SH3-domain-containing proteins cortactin, HS1, and HIP-55 during apoptosis. | Chen, YR., et al. 2001. Biochem Biophys Res Commun. 288: 981-9. PMID: 11689006
- Mammalian actin binding protein 1 is essential for endocytosis but not lamellipodia formation: functional analysis by RNA interference. | Mise-Omata, S., et al. 2003. Biochem Biophys Res Commun. 301: 704-10. PMID: 12565838
- The SH3 domain-containing adaptor HIP-55 mediates c-Jun N-terminal kinase activation in T cell receptor signaling. | Han, J., et al. 2003. J Biol Chem. 278: 52195-202. PMID: 14557276
- Recruitment of the actin-binding protein HIP-55 to the immunological synapse regulates T cell receptor signaling and endocytosis. | Le Bras, S., et al. 2004. J Biol Chem. 279: 15550-60. PMID: 14729663
- PRAM-1 potentiates arsenic trioxide-induced JNK activation. | Denis, FM., et al. 2005. J Biol Chem. 280: 9043-8. PMID: 15637062
- HIP-55 is important for T-cell proliferation, cytokine production, and immune responses. | Han, J., et al. 2005. Mol Cell Biol. 25: 6869-78. PMID: 16055701
- Abl-SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP-55. | Le Bras, S., et al. 2007. FEBS Lett. 581: 967-74. PMID: 17306257
- The actin-binding protein Abp1 controls dendritic spine morphology and is important for spine head and synapse formation. | Haeckel, A., et al. 2008. J Neurosci. 28: 10031-44. PMID: 18829961
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIP-55 Antibody (E-11) | sc-398498 | 200 µg/ml | $316.00 | |||
HIP-55 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-530821 | 200 µg Ab; 10 µg BP | $354.00 | |||
HIP-55 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-524367 | 200 µg Ab, 40 µg BP | $354.00 | |||
HIP-55 Antibody (E-11): m-IgG2a BP-HRP Bundle | sc-547601 | 200 µg Ab; 10 µg BP | $354.00 | |||
HIP-55 Antibody (E-11) AC | sc-398498 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HIP-55 Antibody (E-11) HRP | sc-398498 HRP | 200 µg/ml | $316.00 | |||
HIP-55 Antibody (E-11) FITC | sc-398498 FITC | 200 µg/ml | $330.00 | |||
HIP-55 Antibody (E-11) PE | sc-398498 PE | 200 µg/ml | $343.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 488 | sc-398498 AF488 | 200 µg/ml | $357.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 546 | sc-398498 AF546 | 200 µg/ml | $357.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 594 | sc-398498 AF594 | 200 µg/ml | $357.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 647 | sc-398498 AF647 | 200 µg/ml | $357.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 680 | sc-398498 AF680 | 200 µg/ml | $357.00 | |||
HIP-55 Antibody (E-11) Alexa Fluor® 790 | sc-398498 AF790 | 200 µg/ml | $357.00 |