HIC-1 Antibody (H-6) recommended for detection of HIC-1 of mouse, rat and human origin by WB, IP, IF and ELISA

HIC-1 Antibody (H-6): sc-271499

HIC-1 Antibody (H-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-HIC-1 Antibody (H-6) is a mouse monoclonal IgG2b (kappa light chain) HIC-1 antibody provided at 200 µg/ml
    • raised against amino acids 611-733 mapping at the C-terminus of HIC-1 of human origin
    • Anti-HIC-1 Antibody (H-6) is recommended for detection of HIC-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HIC-1 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-271499 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HIC-1 (H-6): sc-271499.
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Can HIC-1 (H-6): sc-271499 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HIC-1 (H-6): sc-271499 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Clear WB detection This antibody performs well in WB. It will produce clear and specific bands in mouse, rat, and human samples and has been used to publish.
Date published: 2017-02-02
Rated 5 out of 5 by from Great Western Blot data of HIC Great Western Blot data of HIC-1 expression in Jurkat, HeLa and A549 nuclear extracts. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Good Western Blot data of HIC Good Western Blot data of HIC-1 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2014-10-20
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