HGTD-P Antibody (H-4): sc-514243

HGTD-P Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects HGTD-P of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HGTD-P (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HGTD-P, also known as human growth and transformation-dependent protein, E2IG5, or FAM162A, is a 154 amino acid single-pass membrane protein that belongs to the UPF0389 family. HGTD-P functions as a proapoptotic factor, particularly in hypoxia-ischemia (HI), where HGTD-P acts as an effector of cell death. HGTD-P localizes to mitochondria, and HGTD-P overexpression can induce mitochondrial permeability transition by interacting with voltage-dependent anion channels, facilitating apoptotic cell death through mitochondrial apoptotic pathways, including permeability transition, cytochrome c release, and caspase 9 activation. HGTD-P regulation is intricately linked to hypoxia, as hypoxia-inducible factor 1-alpha (HIF-1α) activates HGTD-P through a hypoxia-responsive element located in the promoter region of the HGTD-P gene, making HGTD-P a potential therapeutic target for conditions associated with HI-induced brain damage.

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HGTD-P Antibody (H-4) References:

1. Identification of the hypoxia-inducible factor 1 alpha-responsive HGTD-P gene as a mediator in the mitochondrial apoptotic pathway.  |  Lee, MJ., et al. 2004. Mol Cell Biol. 24: 3918-27. PMID: 15082785
2. Interaction of pro-apoptotic protein HGTD-P with heat shock protein 90 is required for induction of mitochondrial apoptotic cascades.  |  Kim, JY., et al. 2006. FEBS Lett. 580: 3270-5. PMID: 16698020
3. Redox stress and the contributions of BH3-only proteins to infarction.  |  Webster, KA., et al. 2006. Antioxid Redox Signal. 8: 1667-76. PMID: 16987020
4. mHGTD-P mediates hypoxic neuronal cell death via the release of apoptosis-inducing factor.  |  Cho, YE., et al. 2007. Neurosci Lett. 416: 144-9. PMID: 17316997
5. Expression and prognostic significance of human growth and transformation-dependent protein in gastric carcinoma and gastric adenoma.  |  Cho, YE., et al. 2009. Hum Pathol. 40: 975-81. PMID: 19269009
6. Proapoptotic role of human growth and transformation-dependent protein in the developing rat brain after hypoxia-ischemia.  |  Qu, Y., et al. 2009. Stroke. 40: 2843-8. PMID: 19520982
7. In vitro effects of hypoxia-inducible factor 1alpha on the biological characteristics of the SiHa uterine cervix cancer cell line.  |  Tang, B., et al. 2009. Int J Gynecol Cancer. 19: 898-904. PMID: 19574782
8. Expression and clinicopathological significance of human growth and transformation-dependent protein (HGTD-P) in uterine cervical cancer.  |  Cho, YE., et al. 2010. Histopathology. 57: 479-82. PMID: 20840676
9. MiR-139-5p inhibits HGTD-P and regulates neuronal apoptosis induced by hypoxia-ischemia in neonatal rats.  |  Qu, Y., et al. 2014. Neurobiol Dis. 63: 184-93. PMID: 24333693
10. Hypoxic regulation of the expression of genes encoded estrogen related proteins in U87 glioma cells: eff ect of IRE1 inhibition.  |  Minchenko, DO., et al. 2017. Endocr Regul. 51: 8-19. PMID: 28222026
11. Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.  |  Lee, SH., et al. 2020. Sci Data. 7: 425. PMID: 33262348
12. Mechanism of Dcp2/RNCR3/Dkc1/Snora62 axis regulating neuronal apoptosis in chronic cerebral ischemia.  |  Yang, J., et al. 2023. Cell Biol Toxicol. 39: 2881-2898. PMID: 37097350

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HGTD-P Antibody (H-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HGTD-P Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HGTD-P Antibody (H-4) Specifications

Alternate Product Name: HGTD-P

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 77-146

Epitope Target: HGTD-P

Epitope Species: human

Product Reactivity/Detection: HGTD-P

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HGTD-P Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin