Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HES2 CRISPR/Cas9 KO Plasmid (h): sc-407494
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HES2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HES2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HES2 Antibody (H-8): sc-514711
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES2 CRISPR/Cas9 KO Plasmid (h) | sc-407494 | 20 µg | $397.00 |
Overview
HES2 encodes a basic helix-loop-helix (bHLH) transcriptional repressor in the Hairy/Enhancer-of-split family that modulates gene expression programs controlling cell fate decisions. As a downstream effector of Notch signaling, HES2 contributes to transcriptional feedback networks that restrain differentiation and help coordinate developmental timing, frequently acting through promoter-bound repression and interaction with corepressor complexes. By influencing lineage commitment and proliferation–differentiation balance, altered HES2 activity is relevant to studies of developmental disorders and cancer-associated dysregulation of Notch-dependent transcriptional circuitry. In vitro, HES2 perturbation is commonly used to interrogate transcriptional control of progenitor maintenance, neurogenic programs, and context-specific differentiation outputs.
HES2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HES2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HES2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HES2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HES2 protein expression.
This CRISPR knockout system enables efficient generation of HES2-deficient cell models for investigation of HES2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HES2 exon(s) critical for HES2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HES2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HES2 CRISPR/Cas9 KO Plasmid (h) and HES2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HES2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HES2 HDR Plasmid (h) and HES2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HES2 homology arms to support homology-directed repair at defined HES2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.