HES2 Antibody (H-8): sc-514711
- HES2 Antibody (H-8) is a mouse monoclonal IgG1 κ HES2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-17 at the N-terminus of HES2 of human origin
- HES2 Antibody (H-8) is recommended for detection of HES2 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HES2 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514711 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HES2 Antibody (H-8) for WB applications. These reagents are now offered in bundles with HES2 Antibody (H-8) (see ordering information below).
HES2 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HES2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HES2 (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HES2 protein plays a crucial role in gene expression regulation during embryonic development, particularly in the Notch signaling pathway, which is essential for cell differentiation and tissue patterning. HES2 protein is a member of the basic helix-loop-helix (bHLH) transcription factor family, known for binding to specific DNA sequences and regulating target gene transcription. HES2 protein, along with other HES family members, forms a complex with TLE proteins, which are involved in transcriptional repression. HES2-TLE interaction is mediated by the carboxy-terminal WRPW motif, allowing effective inhibition of transcriptional activator activity. This regulatory mechanism maintains balance between cell proliferation and differentiation, particularly in neural and muscle development. HES2 protein expression occurs in various adult and embryonic tissues, highlighting developmental process importance and potential implications in developmental disorders and cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HES2 Antibody (H-8) References:
- The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation. | Bae, S., et al. 2000. Development. 127: 2933-43. PMID: 10851137
- Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. | Sasai, Y., et al. 1992. Genes Dev. 6: 2620-34. PMID: 1340473
- Molecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system. | Akazawa, C., et al. 1992. J Biol Chem. 267: 21879-85. PMID: 1400497
- Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements. | Takebayashi, K., et al. 1994. J Biol Chem. 269: 5150-6. PMID: 7906273
- Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split. | Ishibashi, M., et al. 1993. Eur J Biochem. 215: 645-52. PMID: 8354270
- The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain. | Fisher, AL., et al. 1996. Mol Cell Biol. 16: 2670-7. PMID: 8649374
- Molecular interaction between TLE1 and the carboxyl-terminal domain of HES-1 containing the WRPW motif. | Grbavec, D. and Stifani, S. 1996. Biochem Biophys Res Commun. 223: 701-5. PMID: 8687460
- Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning. | Lobe, CG. 1997. Mech Dev. 62: 227-37. PMID: 9152013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES2 Antibody (H-8) | sc-514711 | 200 µg/ml | $322.00 | |||
HES2 Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-527932 | 200 µg Ab; 10 µg BP | $361.00 | |||
HES2 Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-533305 | 200 µg Ab; 20 µg BP | $361.00 | |||
HES2 Antibody (H-8) X | sc-514711 X | 200 µg/0.1 ml | $322.00 | |||
HES2 Antibody (H-8) AC | sc-514711 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HES2 Antibody (H-8) HRP | sc-514711 HRP | 200 µg/ml | $322.00 | |||
HES2 Antibody (H-8) FITC | sc-514711 FITC | 200 µg/ml | $336.00 | |||
HES2 Antibody (H-8) PE | sc-514711 PE | 200 µg/ml | $349.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 488 | sc-514711 AF488 | 200 µg/ml | $364.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 546 | sc-514711 AF546 | 200 µg/ml | $364.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 594 | sc-514711 AF594 | 200 µg/ml | $364.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 647 | sc-514711 AF647 | 200 µg/ml | $364.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 680 | sc-514711 AF680 | 200 µg/ml | $364.00 | |||
HES2 Antibody (H-8) Alexa Fluor® 790 | sc-514711 AF790 | 200 µg/ml | $364.00 | |||
HES2 (H-8) Neutralizing Peptide | sc-514711 P | 100 µg/0.5 ml | $69.00 |