HERP Antibody (19-Y): sc-100721

HERP Antibody (19-Y) is a mouse monoclonal IgG2a kappa light chain antibody that detects HERP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-HERP antibody (19-Y) is available as the non-conjugated form. HERP protein, also known as HERPUD1 (homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1), plays a crucial role in the endoplasmic reticulum (ER) stress response, which is vital for maintaining cellular homeostasis. This 391 amino acid multi-pass membrane protein is primarily localized to the ER, where HERP (19-Y) monoclonal antibody is involved in the degradation of misfolded proteins through the ER-associated protein degradation (ERAD) system. The presence of an N-terminal ubiquitin-like domain in HERP suggests involvement in tagging these misfolded proteins for destruction, thereby preventing the accumulation of potentially toxic proteins that can disrupt cellular function. HERP is widely expressed, with highest levels found in the brain, and exists in three isoforms due to alternative splicing events, which may contribute to functional diversity in different tissues. Using HERP (19-Y) antibody in research can provide insights into the mechanisms of ER stress and implications in various diseases, including neurodegenerative disorders and metabolic syndromes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HERP Antibody (19-Y) References:

1. The novel MMS-inducible gene Mif1/KIAA0025 is a target of the unfolded protein response pathway.  |  van Laar, T., et al. 2000. FEBS Lett. 469: 123-31. PMID: 10708769
2. Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress.  |  Kokame, K., et al. 2000. J Biol Chem. 275: 32846-53. PMID: 10922362
3. Identification of ERSE-II, a new cis-acting element responsible for the ATF6-dependent mammalian unfolded protein response.  |  Kokame, K., et al. 2001. J Biol Chem. 276: 9199-205. PMID: 11112790
4. Endoplasmic reticulum stress-inducible protein, Herp, enhances presenilin-mediated generation of amyloid beta-protein.  |  Sai, X., et al. 2002. J Biol Chem. 277: 12915-20. PMID: 11799129
5. The ubiquitin-domain protein HERP forms a complex with components of the endoplasmic reticulum associated degradation pathway.  |  Schulze, A., et al. 2005. J Mol Biol. 354: 1021-7. PMID: 16289116
6. Luman/CREB3 induces transcription of the endoplasmic reticulum (ER) stress response protein Herp through an ER stress response element.  |  Liang, G., et al. 2006. Mol Cell Biol. 26: 7999-8010. PMID: 16940180
7. The ubiquitin E3 ligase POSH regulates calcium homeostasis through spatial control of Herp.  |  Tuvia, S., et al. 2007. J Cell Biol. 177: 51-61. PMID: 17420289
8. Characterization of an ERAD pathway for nonglycosylated BiP substrates, which require Herp.  |  Okuda-Shimizu, Y. and Hendershot, LM. 2007. Mol Cell. 28: 544-54. PMID: 18042451
9. Herp enhances ER-associated protein degradation by recruiting ubiquilins.  |  Kim, TY., et al. 2008. Biochem Biophys Res Commun. 369: 741-6. PMID: 18307982
10. Herp depletion inhibits zearalenone-induced cell death in RAW 264.7 macrophages.  |  Chen, F., et al. 2016. Toxicol In Vitro. 32: 115-22. PMID: 26723276
11. Herp Promotes Degradation of Mutant Huntingtin: Involvement of the Proteasome and Molecular Chaperones.  |  Luo, H., et al. 2018. Mol Neurobiol. 55: 7652-7668. PMID: 29430620
12. Herp regulates intracellular survival of Mycobacterium tuberculosis H37Ra in macrophages by regulating reactive oxygen species-mediated autophagy.  |  Son, S-H., et al. 2023. mBio. 14: e0153523. PMID: 37800958

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HERP Antibody (19-Y) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HERP Antibody (19-Y) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HERP Antibody (19-Y) Specifications

Alternate Product Name: HERP

Product Clone Name: 19-Y

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 74-181

Epitope Target: HERP

Epitope Species: human

Product Reactivity/Detection: HERP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HERP Antibody (19-Y): N/A