Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HepaCAM CRISPR/Cas9 KO Plasmid (h): sc-406036
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HepaCAM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HepaCAM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HepaCAM Antibody (B-7): sc-515637
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HepaCAM CRISPR/Cas9 KO Plasmid (h) | sc-406036 | 20 µg | $397.00 |
Overview
HEPACAM encodes HepaCAM, an immunoglobulin-like cell adhesion molecule enriched at the plasma membrane that supports cell–cell interactions and epithelial tissue organization. HepaCAM contributes to contact inhibition, modulation of cell morphology, and regulation of cytoskeletal dynamics through adhesion-associated signaling networks that influence migration and proliferation. Altered HEPACAM expression or function has been linked to dysregulated growth control and invasive behavior in multiple tumor contexts, consistent with roles in maintaining differentiated cellular states. These properties make HEPACAM a useful node for interrogating adhesion-dependent signaling, junctional stability, and phenotypes associated with cellular plasticity.
HepaCAM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HEPACAM gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HEPACAM together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HEPACAM open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HepaCAM protein expression.
This CRISPR knockout system enables efficient generation of HEPACAM-deficient cell models for investigation of HepaCAM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HEPACAM exon(s) critical for HepaCAM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HEPACAM genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HepaCAM CRISPR/Cas9 KO Plasmid (h) and HepaCAM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HEPACAM locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HepaCAM HDR Plasmid (h) and HepaCAM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HEPACAM homology arms to support homology-directed repair at defined HEPACAM target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.