Date published: 2025-11-18

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Hemogen Antibody (C-7): sc-393416

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Datasheets
  • Hemogen Antibody (C-7) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of Hemogen of human origin
  • Anti-Hemogen Antibody (C-7) is recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-Hemogen Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Hemogen Antibody (C-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Hemogen Antibody (C-7) (see ordering information below).

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    Hemogen Antibody (C-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Hemogen in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Hemogen, also known as hemopoietic gene protein or erythroid differentiation-associated gene protein, is a crucial 484 amino acid nuclear protein encoded by the human gene HEMGN. Hemogen (C-7) antibody recognizes a protein that plays a significant role in hematopoiesis, particularly in erythroid cell differentiation, vital for red blood cell production. Hemogen is predominantly expressed in hematopoietic precursor cells and can be detected in various tissues and cell lines, including CD34+ cells and K-562 leukemia cell line, as well as in bone marrow, testis, thymus, and thyroid tumors, along with non-Hodgkin lymphoma and various leukemia cell lines. Hemogen expression is tightly regulated; down-regulated during megakaryocytic differentiation of K-562 cells in response to 12-O-tetradecanoylphorbol-13-acetate, while up-regulated in normal peripheral blood mononuclear cells by mitogenic stimulation. anti-Hemogen antibody (C-7) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, making this research tool versatile for studying hematopoietic processes and related pathologies.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Hemogen Antibody (C-7)

    sc-393416
    200 µg/ml
    $316.00

    Hemogen Antibody (C-7): m-IgG Fc BP-HRP Bundle

    sc-526249
    200 µg Ab; 10 µg BP
    $354.00

    Hemogen Antibody (C-7): m-IgG2a BP-HRP Bundle

    sc-546540
    200 µg Ab; 10 µg BP
    $354.00

    Hemogen Antibody (C-7) AC

    sc-393416 AC
    500 µg/ml, 25% agarose
    $416.00

    Hemogen Antibody (C-7) HRP

    sc-393416 HRP
    200 µg/ml
    $316.00

    Hemogen Antibody (C-7) FITC

    sc-393416 FITC
    200 µg/ml
    $330.00

    Hemogen Antibody (C-7) PE

    sc-393416 PE
    200 µg/ml
    $343.00

    Hemogen Antibody (C-7) Alexa Fluor® 488

    sc-393416 AF488
    200 µg/ml
    $357.00

    Hemogen Antibody (C-7) Alexa Fluor® 546

    sc-393416 AF546
    200 µg/ml
    $357.00

    Hemogen Antibody (C-7) Alexa Fluor® 594

    sc-393416 AF594
    200 µg/ml
    $357.00

    Hemogen Antibody (C-7) Alexa Fluor® 647

    sc-393416 AF647
    200 µg/ml
    $357.00

    Hemogen Antibody (C-7) Alexa Fluor® 680

    sc-393416 AF680
    200 µg/ml
    $357.00

    Hemogen Antibody (C-7) Alexa Fluor® 790

    sc-393416 AF790
    200 µg/ml
    $357.00

    Can Hemogen (C-7): sc-393416 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Randy McDonald
    Thank you for your question. Yes, Hemogen (C-7): sc-393416 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 5 out of 5 by from Good Western blot data of Hemogen expression in KGood Western blot data of Hemogen expression in K-562 and Jurkat nuclear extracts. -SCBT QC
    Date published: 2013-06-20
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    Hemogen Antibody (C-7) is rated 5.0 out of 5 by 1.
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