Hemogen Antibody (G-2): sc-377438
- Hemogen Antibody (G-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 181-360 mapping within an internal region of Hemogen of mouse origin
- Anti-Hemogen Antibody (G-2) is recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Hemogen Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hemogen Antibody (G-2) for WB applications. These reagents are now offered in bundles with Hemogen Antibody (G-2) (see ordering information below).
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Hemogen Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hemogen in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Hemogen antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hemogen, also known as hemopoietic gene protein or erythroid differentiation-associated gene protein, is a 484 amino acid nuclear protein encoded by HEMGN. Hemogen (G-2) antibody targets a protein that plays a crucial role in hematopoiesis, particularly in erythroid cell differentiation, which is vital for red blood cell production. Hemogen expression is primarily observed in hematopoietic precursor cells, including CD34+ cells and K-562 leukemia cell line, as well as in bone marrow, testis, thymus, and thyroid tumors. Hemogen (G-2) monoclonal antibody recognizes a protein implicated in non-Hodgkin lymphoma and various leukemia cell lines, with expression modulated by external factors; for instance, Hemogen is down-regulated during megakaryocytic differentiation in K-562 cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) at protein level, while up-regulated in normal peripheral blood mononuclear cells by mitogens. This dynamic regulation of Hemogen underscores its importance in both normal hematopoiesis and disease states, making anti-Hemogen antibody (G-2) a valuable research tool.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hemogen Antibody (G-2) References:
- Hemogen is a novel nuclear factor specifically expressed in mouse hematopoietic development and its human homologue EDAG maps to chromosome 9q22, a region containing breakpoints of hematological neoplasms. | Yang, LV., et al. 2001. Mech Dev. 104: 105-11. PMID: 11404085
- [Overexpression of EDAG-1 in NIH3T3 cells leads to malignant transformation]. | Lü, J., et al. 2002. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 34: 95-8. PMID: 11958143
- Isolation and Characterization of EDAG-1, A Novel Gene Related to Regulation in Hematopoietic System. | Lü, J., et al. 2001. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 33: 641-646. PMID: 12035055
- Alternative promoters and polyadenylation regulate tissue-specific expression of Hemogen isoforms during hematopoiesis and spermatogenesis. | Yang, LV., et al. 2003. Dev Dyn. 228: 606-16. PMID: 14648837
- EDAG regulates the proliferation and differentiation of hematopoietic cells and resists cell apoptosis through the activation of nuclear factor-kappa B. | Li, CY., et al. 2004. Cell Death Differ. 11: 1299-308. PMID: 15332117
- High expression of EDAG and its significance in AML. | An, LL., et al. 2005. Leukemia. 19: 1499-502. PMID: 15920494
- The GATA site-dependent hemogen promoter is transcriptionally regulated by GATA1 in hematopoietic and leukemia cells. | Yang, LV., et al. 2006. Leukemia. 20: 417-25. PMID: 16437149
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hemogen Antibody (G-2) | sc-377438 | 200 µg/ml | $316.00 | |||
Hemogen Antibody (G-2): m-IgG Fc BP-HRP Bundle | sc-527603 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hemogen Antibody (G-2): m-IgG1 BP-HRP Bundle | sc-532976 | 200 µg Ab; 20 µg BP | $354.00 | |||
Hemogen Antibody (G-2) AC | sc-377438 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Hemogen Antibody (G-2) HRP | sc-377438 HRP | 200 µg/ml | $316.00 | |||
Hemogen Antibody (G-2) FITC | sc-377438 FITC | 200 µg/ml | $330.00 | |||
Hemogen Antibody (G-2) PE | sc-377438 PE | 200 µg/ml | $343.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 488 | sc-377438 AF488 | 200 µg/ml | $357.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 546 | sc-377438 AF546 | 200 µg/ml | $357.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 594 | sc-377438 AF594 | 200 µg/ml | $357.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 647 | sc-377438 AF647 | 200 µg/ml | $357.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 680 | sc-377438 AF680 | 200 µg/ml | $357.00 | |||
Hemogen Antibody (G-2) Alexa Fluor® 790 | sc-377438 AF790 | 200 µg/ml | $357.00 |